Calcium Dependency and Contractility in TNT-Connected C2C12 Cells Responding to Mechanical Stimulation.

IF 3.5 4区医学 Q3 BIOPHYSICS

Cellular and molecular bioengineering Pub Date : 2026-05-15 eCollection Date: 2026-06-01 DOI:10.1007/s12195-026-00908-y

Yanli Sun, Hucheng Zhao

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引用次数: 0

Abstract

Purpose: Tunneling nanotubes (TNTs) are membrane-based intercellular structures that support long-range communication between spatially separated cells. Although TNT-associated electrical responses have been observed under mechanical stimulation, whether these responses are linked to changes in cellular mechanical behavior remains unclear.

Methods: Mechanical stimulation was applied to one cell within TNT-connected C2C12 pairs. Traction force and cell spreading area in the connected cell were evaluated under different pharmacological conditions to investigate the involvement of calcium signaling, calcium channels, gap junction communication, and cellular contractility.

Results: Mechanical stimulation increased traction force and reduced cell spreading area in TNT-connected cells. These responses were suppressed by calcium depletion, inhibition of T-type calcium channels, or blockade of gap junction communication, but were not significantly affected by inhibition of L-type calcium channels.

Conclusions: These findings support a calcium-dependent association between TNT-mediated intercellular signaling and biomechanical regulation in connected C2C12 cells.

Supplementary information: The online version contains supplementary material available at 10.1007/s12195-026-00908-y.

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tnt连接的C2C12细胞对机械刺激的钙依赖性和收缩性

目的：隧道纳米管（TNTs）是一种基于膜的细胞间结构，支持空间分离细胞之间的远程通信。尽管在机械刺激下已经观察到与tnt相关的电反应，但这些反应是否与细胞力学行为的变化有关仍不清楚。方法：采用机械刺激的方法，对连接tnt的C2C12对细胞中的一个细胞进行机械刺激。在不同的药理学条件下，评估连接细胞的牵引力和细胞扩张面积，以研究钙信号、钙通道、间隙连接通信和细胞收缩性的参与。结果：机械刺激增加了tnt连接细胞的牵引力，减少了细胞扩散面积。这些反应被钙耗尽、t型钙通道的抑制或间隙连接通讯的阻断所抑制，而l型钙通道的抑制对这些反应没有显著影响。结论：这些发现支持在连接的C2C12细胞中，tnt介导的细胞间信号传导和生物力学调节之间存在钙依赖性关联。补充信息：在线版本包含补充资料，提供地址为10.1007/s12195-026-00908-y。

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来源期刊

Cellular and molecular bioengineering 生物-生物物理

CiteScore

5.60

自引率

3.60%

发文量

审稿时长

>12 weeks

期刊介绍： The field of cellular and molecular bioengineering seeks to understand, so that we may ultimately control, the mechanical, chemical, and electrical processes of the cell. A key challenge in improving human health is to understand how cellular behavior arises from molecular-level interactions. CMBE, an official journal of the Biomedical Engineering Society, publishes original research and review papers in the following seven general areas: Molecular: DNA-protein/RNA-protein interactions, protein folding and function, protein-protein and receptor-ligand interactions, lipids, polysaccharides, molecular motors, and the biophysics of macromolecules that function as therapeutics or engineered matrices, for example. Cellular: Studies of how cells sense physicochemical events surrounding and within cells, and how cells transduce these events into biological responses. Specific cell processes of interest include cell growth, differentiation, migration, signal transduction, protein secretion and transport, gene expression and regulation, and cell-matrix interactions. Mechanobiology: The mechanical properties of cells and biomolecules, cellular/molecular force generation and adhesion, the response of cells to their mechanical microenvironment, and mechanotransduction in response to various physical forces such as fluid shear stress. Nanomedicine: The engineering of nanoparticles for advanced drug delivery and molecular imaging applications, with particular focus on the interaction of such particles with living cells. Also, the application of nanostructured materials to control the behavior of cells and biomolecules.