Neonatal and Adult Dermal Fibroblasts Remodel Hyaluronan Enriched Pericellular Matrix Differentially in Hyaluronic Acid Binding Peptide-Tethered Collagen Hydrogels.

IF 3.5 4区医学 Q3 BIOPHYSICS

Cellular and molecular bioengineering Pub Date : 2026-04-25 eCollection Date: 2026-06-01 DOI:10.1007/s12195-026-00907-z

Vanessa Christine Smith, Whitney Ann Ponwith, Steve Smith, Tugba Ozdemir

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引用次数: 0

Abstract

Purpose: The goal of this study was to evaluate the impact of hyaluronic acid binding peptide (HABP) on pericellular matrix remodeling and to determine whether this effect differs between adult and neonatal fibroblasts. Neonatal wound healing is characterized by minimal scarring compared to adult healing, which typically results in fibrotic scar formation. Hyaluronic acid (HA) plays an important role in extracellular matrix organization and collagen remodeling during scarless healing. We hypothesized that HABP would promote endogenous HA retention around the pericellular matrix and influence collagen remodeling in fibroblasts.

Methods: Adult and neonatal human dermal fibroblasts were cultured in both two-dimensional and three-dimensional environments. HABP-tethered surfaces were used to promote HA retention around cells. Pericellular matrix formation and cell morphology were assessed in two-dimensional culture, while collagen contraction and collagen fibril morphology were evaluated in three-dimensional collagen gel systems.

Results: Adult fibroblasts cultured on HABP surfaces appeared similar in shape to neonatal fibroblasts, suggesting that HABP could promote a more regenerative phenotype. Collagen contraction was steeper in peptide-tethered conditions than in control samples for both cell types. The presence of HABP increased contraction significantly in the presence of Hyaluronidase (HAdase). Collagen fibers exhibited altered width and organization in the presence of HABP and Scr-HABP compared with control samples.

Conclusions: These findings suggest that HABP enhances HA retention and alters collagen remodeling, supporting a pro-regenerative fibroblast remodeling response.

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新生儿和成人真皮成纤维细胞在透明质酸结合肽系系胶原水凝胶中重塑富含透明质酸的细胞周围基质的差异。

目的：本研究的目的是评估透明质酸结合肽（HABP）对细胞周围基质重塑的影响，并确定这种影响在成人和新生儿成纤维细胞之间是否不同。新生儿伤口愈合的特点是最小的疤痕相比，成人愈合，这通常导致纤维化瘢痕形成。透明质酸（HA）在无疤痕愈合过程中对细胞外基质组织和胶原蛋白重塑起着重要作用。我们假设HABP会促进细胞周基质周围的内源性HA保留并影响成纤维细胞中的胶原重塑。方法：分别在二维和三维环境下培养成人和新生儿真皮成纤维细胞。habp系固表面用于促进HA在细胞周围的保留。在二维培养中评估细胞周围基质形成和细胞形态，而在三维胶原凝胶体系中评估胶原收缩和胶原纤维形态。结果：在HABP表面培养的成纤维细胞在形状上与新生儿成纤维细胞相似，表明HABP可以促进更再生的表型。两种细胞类型的胶原蛋白在肽系固条件下比对照样品收缩更剧烈。在透明质酸酶（haase）存在时，HABP显著增加收缩。与对照样品相比，在HABP和Scr-HABP存在下，胶原纤维的宽度和组织发生了改变。结论：这些研究结果表明，HABP增强HA保留并改变胶原重塑，支持促再生成纤维细胞重塑反应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cellular and molecular bioengineering 生物-生物物理

CiteScore

5.60

自引率

3.60%

发文量

审稿时长

>12 weeks

期刊介绍： The field of cellular and molecular bioengineering seeks to understand, so that we may ultimately control, the mechanical, chemical, and electrical processes of the cell. A key challenge in improving human health is to understand how cellular behavior arises from molecular-level interactions. CMBE, an official journal of the Biomedical Engineering Society, publishes original research and review papers in the following seven general areas: Molecular: DNA-protein/RNA-protein interactions, protein folding and function, protein-protein and receptor-ligand interactions, lipids, polysaccharides, molecular motors, and the biophysics of macromolecules that function as therapeutics or engineered matrices, for example. Cellular: Studies of how cells sense physicochemical events surrounding and within cells, and how cells transduce these events into biological responses. Specific cell processes of interest include cell growth, differentiation, migration, signal transduction, protein secretion and transport, gene expression and regulation, and cell-matrix interactions. Mechanobiology: The mechanical properties of cells and biomolecules, cellular/molecular force generation and adhesion, the response of cells to their mechanical microenvironment, and mechanotransduction in response to various physical forces such as fluid shear stress. Nanomedicine: The engineering of nanoparticles for advanced drug delivery and molecular imaging applications, with particular focus on the interaction of such particles with living cells. Also, the application of nanostructured materials to control the behavior of cells and biomolecules.