Antioxidant potential and metabolic profiling of Viola odorata and the endophytic Rhizobium sp. BR7.

IF 2.5 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology Letters Pub Date : 2026-07-15 DOI:10.1007/s10529-026-03764-y

Richa Salwan, Vivek Sharma, Himanshu Dwivedi, Abdul Qayum, R Murugeswaran

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引用次数: 0

Abstract

The interaction between medicinal plants and their root-associated microbes plays a key role in shaping rhizosphere chemistry and bioactivity. This study evaluates the antioxidant potential and metabolic profiles of Viola odorata roots and associated Rhizobium sp. BR7 using in vitro DPPH, ABTS, FRAC based antioxidant assays and LC-MS-based untargeted metabolomics. Both plant and microbial extracts exhibited significant free radical-scavenging capacity. The isolate BR7 showed the most potent antioxidant activity (IC₅₀ = 69 µg/mL), followed by AH3, JN9, and SGA9. Isolates BR5 and KB11 showed lower efficacy. The inverse correlation was observed between flavonoid content and ABTS based antioxidant activity, indicating the key role of flavonoids in antioxidant potential. LC-MS based analysis revealed overlapping antioxidant metabolites such as caffeic acid derivatives, kaempferol-3-O46 glucoside, apigenin, and chlorogenic acid, indicating metabolic complementarity between plant and bacteria. Besides these, BR7 also contained unique compounds including indole-3-acetic acid (IAA) fragments, rhamnolipid-like biosurfactants, and alkylresorcinols, suggesting strong rhizosphere adaptability. Overall, Rhizobium sp. BR7 emerges as a promising source of antioxidant co-metabolites with potential applications in biopharmaceutical and functional food development.

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堇菜和内生根瘤菌BR7的抗氧化潜能和代谢特征。

药用植物与其根相关微生物之间的相互作用在形成根际化学和生物活性方面起着关键作用。本研究利用体外DPPH、ABTS、FRAC抗氧化测定和lc - ms非靶向代谢组学方法，对堇菜根及其伴生根瘤菌BR7的抗氧化潜力和代谢谱进行了评价。植物提取物和微生物提取物均表现出显著的自由基清除能力。BR7的抗氧化活性最强（IC50 = 69µg/mL），其次是AH3、JN9和SGA9。分离株BR5和KB11的疗效较低。黄酮类化合物含量与ABTS抗氧化活性呈负相关，表明黄酮类化合物在抗氧化能力中起关键作用。基于LC-MS的分析发现了重叠的抗氧化代谢产物，如咖啡酸衍生物、山奈酚-3- o46葡萄糖苷、芹菜素和绿原酸，表明植物和细菌之间的代谢互补。此外，BR7还含有吲哚-3-乙酸（IAA）片段、鼠李糖脂类生物表面活性剂和烷基间苯二酚等独特化合物，具有较强的根际适应性。总之，根瘤菌BR7是一种很有前景的抗氧化共代谢产物，在生物制药和功能食品开发中具有潜在的应用前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnology Letters 工程技术-生物工程与应用微生物

CiteScore

5.90

自引率

3.70%

发文量

108

审稿时长

1.2 months

期刊介绍： Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.