Combined Effects of Dinotefuran and Piperonyl Butoxide on Behavioral Development in an F1-Generation Toxicity Study in Mice

IF 2 4区医学 Q4 DEVELOPMENTAL BIOLOGY

Birth Defects Research Pub Date : 2026-07-08 DOI:10.1002/bdr2.70086

Toyohito Tanaka, Motoki Hojo, Akiko Inomata

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引用次数: 0

Abstract

Background

Few published studies have reported on the reproductive and neurobehavioral toxicity of combined exposure to neonicotinoid insecticides and synergists in mammals. This study aimed to evaluate the reproductive and neurobehavioral effects of a combined treatment with dinotefuran (DIN) and piperonyl butoxide (PBO) in an F₁-generation toxicity study in mice.

Methods

DIN and PBO were given in the diet to provide levels of 0% (control), DIN 0.005% + PBO 0.03%, DIN 0.01% + PBO 0.03%, and DIN 0.02% + PBO 0.03% from 5 weeks of age of the F₀ generation to 11 weeks of age of the F₁ generation in mice. Selected reproductive and neurobehavioral parameters were measured in the F₁ generation.

Results

For behavioral development during the lactation period, surface righting on PND 4 and olfactory orientation route on PND 14 indicated a significantly high score in the DIN 0.02% + PBO 0.03% group in male offspring. In female offspring, olfactory orientation route and time on PND 14 indicated a significantly high score in the DIN 0.02% + PBO 0.03% group. For exploratory behavior in the F₁-generation offspring, several variables indicated significant effects in male and female offspring. In the spontaneous behavior of males in the F₁ generation, the parallel lines among the control and treatment groups indicated significant distances in total distance, movement time, and number of rearing.

Conclusions

The dose levels of DIN with PBO in the present study produced several adverse effects on neurobehavioral parameters in F₁ generation mice at lower dose levels than DIN alone.

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敌敌呋喃和丁酰胡椒酯对小鼠f1代行为发育的联合毒性研究。

背景：关于新烟碱类杀虫剂和增效剂联合暴露对哺乳动物的生殖和神经行为毒性的报道很少。在f1代小鼠毒性研究中，本研究旨在评估双呋喃（DIN）和胡椒酰丁醇（PBO）联合治疗对小鼠生殖和神经行为的影响。方法：在F0代5周龄至F1代11周龄的小鼠饲粮中分别添加0%（对照）、0.005% + PBO 0.03%、0.01% + PBO 0.03%、0.02% + PBO 0.03%水平的DIN和PBO。在F1代中测量了选择的生殖和神经行为参数。结果：在哺乳期行为发育方面，雄性子代PND 4的表面矫直和PND 14的嗅觉定向路线在DIN 0.02% + PBO 0.03%组得分显著较高。在雌性后代中，嗅觉定向路线和PND 14上的时间在DIN 0.02% + PBO 0.03%组中得分显著较高。对于f1代后代的探索行为，有几个变量表明雄性和雌性后代都有显著影响。在F1代雄性的自发行为中，对照组和处理组的平行线在总距离、运动时间和饲养次数上均有显著差异。结论：本研究中，与单独使用DIN相比，低剂量的DIN与PBO对F1代小鼠的神经行为参数产生了多种不良影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Birth Defects Research Medicine-Embryology

CiteScore

3.60

自引率

9.50%

发文量

153

期刊介绍： The journal Birth Defects Research publishes original research and reviews in areas related to the etiology of adverse developmental and reproductive outcome. In particular the journal is devoted to the publication of original scientific research that contributes to the understanding of the biology of embryonic development and the prenatal causative factors and mechanisms leading to adverse pregnancy outcomes, namely structural and functional birth defects, pregnancy loss, postnatal functional defects in the human population, and to the identification of prenatal factors and biological mechanisms that reduce these risks. Adverse reproductive and developmental outcomes may have genetic, environmental, nutritional or epigenetic causes. Accordingly, the journal Birth Defects Research takes an integrated, multidisciplinary approach in its organization and publication strategy. The journal Birth Defects Research contains separate sections for clinical and molecular teratology, developmental and reproductive toxicology, and reviews in developmental biology to acknowledge and accommodate the integrative nature of research in this field. Each section has a dedicated editor who is a leader in his/her field and who has full editorial authority in his/her area.