Structural evidence of thioacetate reduction by fluorescent protein mRuby

IF 2.3 4区生物学 Q3 BIOPHYSICS

European Biophysics Journal Pub Date : 2026-07-09 DOI:10.1007/s00249-026-01855-2

Jianshu Dong, Jiachong Xie, Qian Cao

引用次数: 0

Abstract

Red fluorescent protein mRuby is an important labelling tool for super-resolution imaging. With the addition of thioacetate, it was found here that the red colour of the protein aqueous solution changed, although still clear and transparent. Light spectrophotometry exhibited that, with the increase of thioacetate concentration, the absorption spectra changed abruptly. Crystallography showed the binding of thioacetate at the chromophore of mRuby. Atomic-resolution serial structures of mRuby single crystal showed snapshots of step-by-step reduction of thioacetate upon light irradiation. This observation suggests new insights for FP-compound interaction research and light energy harvesting for green chemistry.

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荧光蛋白mRuby还原硫乙酸的结构证据。

红色荧光蛋白mRuby是超分辨率成像的重要标记工具。随着硫乙酸酯的加入，我们发现蛋白质水溶液的红色发生了变化，尽管仍然是清澈透明的。光分光光度法表明，随着硫乙酸浓度的增加，吸收光谱发生突变。晶体学显示了硫乙酸盐与mRuby发色团的结合。mRuby单晶的原子分辨率序列结构显示了光照射下硫乙酸逐步还原的快照。这一发现为fp -化合物相互作用研究和绿色化学的光能收集提供了新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

European Biophysics Journal 生物-生物物理

CiteScore

4.30

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The journal publishes papers in the field of biophysics, which is defined as the study of biological phenomena by using physical methods and concepts. Original papers, reviews and Biophysics letters are published. The primary goal of this journal is to advance the understanding of biological structure and function by application of the principles of physical science, and by presenting the work in a biophysical context. Papers employing a distinctively biophysical approach at all levels of biological organisation will be considered, as will both experimental and theoretical studies. The criteria for acceptance are scientific content, originality and relevance to biological systems of current interest and importance. Principal areas of interest include: - Structure and dynamics of biological macromolecules - Membrane biophysics and ion channels - Cell biophysics and organisation - Macromolecular assemblies - Biophysical methods and instrumentation - Advanced microscopics - System dynamics.