Functional characterisation of pigeon egg proteins based on data-independent acquisition quantitative proteomics.

IF 1.7 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

British Poultry Science Pub Date : 2026-07-08 DOI:10.1080/00071668.2026.2685079

R Liu, Z Bu, D Cheng, R Zhang, Q Tang, L Chang

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引用次数: 0

Abstract

1. This study characterised proteomic differences among pigeon, chicken and quail eggs and explored the functional properties of pigeon egg proteins.2. Nine eggs each from White King pigeons (WKP), nine Hy-Line Brown chickens (HLC) and nine Shendan No.1 quail (S1Q) were selected and pooled into three replicates for each species. Data-independent acquisition (DIA)-based quantitative proteomics was employed to identify differentially expressed proteins, followed by bioinformatics analyses.3. A total of 8,835 peptides corresponding to 937 proteins were identified and quantified across nine samples. In the WKPvs.HLC comparison, 423 proteins were differentially expressed, including 178 upregulated and 245 downregulated proteins. In the WKPvs.S1Q comparison, 479 proteins showed significant expression changes, with 190 upregulated and 289 downregulated proteins. Gene Ontology (GO), Kyoto Encyclopaedia of Genes and Genomes (KEGG) enrichment and protein-protein interaction (PPI) network analyses revealed that WKP egg proteins were significantly enriched. This was related to lipid transport and metabolism, immune regulation and antioxidant activity, transparent albumen gel formation and wound healing.4. These results demonstrate distinct proteomic profiles between pigeon eggs and other poultry eggs. Overall, pigeon eggs possess unique functional protein characteristics, providing a molecular basis for their further development and utilisation as a potential functional protein source.

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基于数据独立采集定量蛋白质组学的鸽蛋蛋白功能表征。

1. 本研究对鸽蛋、鸡蛋和鹌鹑蛋的蛋白质组学差异进行了分析，探讨了鸽蛋蛋白质的功能特性。选取白帝鸽（WKP）、海兰褐鸡（HLC）和神丹1号鹌鹑（S1Q）各9只蛋，每种蛋分成3个重复。采用基于数据独立采集（DIA）的定量蛋白质组学方法鉴定差异表达蛋白，然后进行生物信息学分析。在9个样品中，共鉴定和定量了8835个肽，对应937个蛋白质。在WKPvs。hplc比较，423个蛋白存在差异表达，其中上调蛋白178个，下调蛋白245个。在WKPvs。S1Q比较，479个蛋白表达显著改变，190个蛋白表达上调，289个蛋白表达下调。基因本体（GO）、京都基因与基因组百科全书（KEGG）富集和蛋白相互作用（PPI）网络分析显示，WKP蛋蛋白显著富集。这与脂质转运和代谢、免疫调节和抗氧化活性、透明蛋白凝胶形成和伤口愈合有关。这些结果证明了鸽子蛋和其他家禽蛋之间不同的蛋白质组学特征。总之，鸽蛋具有独特的功能蛋白特性，为其作为一种潜在的功能蛋白来源的进一步开发利用提供了分子基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

British Poultry Science 农林科学-奶制品与动物科学

CiteScore

3.90

自引率

5.00%

发文量

审稿时长

4.5 months

期刊介绍： From its first volume in 1960, British Poultry Science has been a leading international journal for poultry scientists and advisers to the poultry industry throughout the world. Over 60% of the independently refereed papers published originate outside the UK. Most typically they report the results of biological studies with an experimental approach which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: anatomy, embryology, biochemistry, biophysics, physiology, reproduction and genetics, behaviour, microbiology, endocrinology, nutrition, environmental science, food science, feeding stuffs and feeding, management and housing welfare, breeding, hatching, poultry meat and egg yields and quality.Papers that adopt a modelling approach or describe the scientific background to new equipment or apparatus directly relevant to the industry are also published. The journal also features rapid publication of Short Communications. Summaries of papers presented at the Spring Meeting of the UK Branch of the WPSA are published in British Poultry Abstracts .