Expression of Transforming Growth Factor Beta 1 in Lung Adenocarcinoma and Its Relationship with Circulating Tumour Cells.

IF 1.9 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical Genetics Pub Date : 2026-07-08 DOI:10.1007/s10528-026-11420-3

Ning Chen, Duo-Jun Qiu, Jian-Zhu Yang, Gui-Yun Zhu, Li-Fei Kang, Xiao-Ying An, Zhi-Ming Xu, Xiao-Xia Li

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Abstract

To investigate the relationship between the expression of transforming growth factor beta 1 (TGF-β1), epithelial-mesenchymal transition (EMT)-related proteins, circulating tumour cells (CTCs) positivity rate and tumour differentiation degree in lung adenocarcinoma. A prospective study was conducted, including 78 patients with lung adenocarcinoma who underwent surgical resection at our hospital between March 2021 and December 2021. Clinical data, lung adenocarcinoma and adjacent tissues were collected. Western blot, immunohistochemistry and RT-PCR were used to detect the expression of TGF-β1, EMT-related proteins (E-cadherin, vimentin) and mRNA in lung adenocarcinoma. Peripheral blood for CTC analysis was collected within 1 week before surgical resection. Pearson correlation analysis was employed to assess the relationship between TGF-β1, EMT and CTCs. There was a significant correlation between the positive rate of CTCs and the degree of tumour differentiation in patients with lung adenocarcinoma (P < 0.05). The expression of TGF-β1 increased significantly as the degree of differentiation decreased (P < 0.05). It was positively correlated with vimentin expression and negatively correlated with E-cadherin expression (P < 0.05). High expression of TGF-β1 in lung adenocarcinoma promotes EMT and the occurrence of CTCs and is associated with the degree of tumour differentiation.

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转化生长因子β 1在肺腺癌组织中的表达及其与循环肿瘤细胞的关系

探讨肺腺癌组织中转化生长因子β1 （TGF-β1）、上皮-间质转化（EMT）相关蛋白表达、循环肿瘤细胞（ctc）阳性率与肿瘤分化程度的关系。前瞻性研究纳入了2021年3月至2021年12月在我院行肺腺癌手术切除的78例患者。收集临床资料、肺腺癌及邻近组织。采用Western blot、免疫组织化学、RT-PCR检测肺腺癌组织中TGF-β1、emt相关蛋白（E-cadherin、vimentin）及mRNA的表达。术前1周内采集外周血进行CTC分析。采用Pearson相关分析评估TGF-β1、EMT与ctc之间的关系。肺腺癌患者ctc阳性率与肿瘤分化程度有显著相关性(P

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来源期刊

Biochemical Genetics 生物-生化与分子生物学

CiteScore

3.90

自引率

0.00%

发文量

133

审稿时长

4.8 months

期刊介绍： Biochemical Genetics welcomes original manuscripts that address and test clear scientific hypotheses, are directed to a broad scientific audience, and clearly contribute to the advancement of the field through the use of sound sampling or experimental design, reliable analytical methodologies and robust statistical analyses. Although studies focusing on particular regions and target organisms are welcome, it is not the journal’s goal to publish essentially descriptive studies that provide results with narrow applicability, or are based on very small samples or pseudoreplication. Rather, Biochemical Genetics welcomes review articles that go beyond summarizing previous publications and create added value through the systematic analysis and critique of the current state of knowledge or by conducting meta-analyses. Methodological articles are also within the scope of Biological Genetics, particularly when new laboratory techniques or computational approaches are fully described and thoroughly compared with the existing benchmark methods. Biochemical Genetics welcomes articles on the following topics: Genomics; Proteomics; Population genetics; Phylogenetics; Metagenomics; Microbial genetics; Genetics and evolution of wild and cultivated plants; Animal genetics and evolution; Human genetics and evolution; Genetic disorders; Genetic markers of diseases; Gene technology and therapy; Experimental and analytical methods; Statistical and computational methods.