{"title":"Detection of Felis catus Gammaherpesvirus 1 in Cats, but not Dogs, in Türkiye: Prevalence and Risk Factors.","authors":"Murat Şevik","doi":"10.1002/vms3.71075","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The first gammaherpesvirus to be identified in cats, Felis catus gammaherpesvirus 1 (FcaGHV1), has been identified in different countries worldwide. However, there are limited studies on FcaGHV1 infection in Türkiye.</p><p><strong>Objectives: </strong>The current study was carried out to determine prevalence of FcaGHV1 in Turkish domestic cats and dogs and to investigate potential risk factors that are associated with the detection of FcaGHV1.</p><p><strong>Methods: </strong>Whole blood samples were collected from cats (n = 54) and dogs (n = 44). The glycoprotein B (gB) gene-based TaqMan real-time PCR assay was utilised to determine the presence of FcaGHV1-specific DNA. Furthermore, the gB gene region of samples that were found positive in the TaqMan real-time PCR assay was amplified through conventional PCR.</p><p><strong>Results: </strong>FcaGHV1-specific DNA was detected in 4 (7.4%, 95% confidence interval 0.4-14.4) of the 54 feline blood samples by TaqMan real-time PCR. However, FcaGHV1-specific DNA was not detected in the EDTA-blood samples from dogs. The field isolates in this study and isolates from domestic cats in Austria, Japan, Panama, Singapore, Switzerland, and the USA were clustered together by the phylogenetic analysis based on gB gene sequences. Analysis of risk factors showed that there was no significant association between the investigated variables (age, sex, breed, health status, neutering status and outdoor access) and FcaGHV1 detection.</p><p><strong>Conclusions: </strong>According to the current study, FcaGHV1 circulates in domestic cats in Türkiye. Furthermore, the results of this study suggest that virus transmission does not occur between cat and dog species. It is necessary to conduct more research to determine the prevalence of FcaGHV1 in Türkiye and to confirm the risk factors associated with FcaGHV1 detection.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"12 4","pages":"e71075"},"PeriodicalIF":2.1000,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13334453/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary Medicine and Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1002/vms3.71075","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Background: The first gammaherpesvirus to be identified in cats, Felis catus gammaherpesvirus 1 (FcaGHV1), has been identified in different countries worldwide. However, there are limited studies on FcaGHV1 infection in Türkiye.
Objectives: The current study was carried out to determine prevalence of FcaGHV1 in Turkish domestic cats and dogs and to investigate potential risk factors that are associated with the detection of FcaGHV1.
Methods: Whole blood samples were collected from cats (n = 54) and dogs (n = 44). The glycoprotein B (gB) gene-based TaqMan real-time PCR assay was utilised to determine the presence of FcaGHV1-specific DNA. Furthermore, the gB gene region of samples that were found positive in the TaqMan real-time PCR assay was amplified through conventional PCR.
Results: FcaGHV1-specific DNA was detected in 4 (7.4%, 95% confidence interval 0.4-14.4) of the 54 feline blood samples by TaqMan real-time PCR. However, FcaGHV1-specific DNA was not detected in the EDTA-blood samples from dogs. The field isolates in this study and isolates from domestic cats in Austria, Japan, Panama, Singapore, Switzerland, and the USA were clustered together by the phylogenetic analysis based on gB gene sequences. Analysis of risk factors showed that there was no significant association between the investigated variables (age, sex, breed, health status, neutering status and outdoor access) and FcaGHV1 detection.
Conclusions: According to the current study, FcaGHV1 circulates in domestic cats in Türkiye. Furthermore, the results of this study suggest that virus transmission does not occur between cat and dog species. It is necessary to conduct more research to determine the prevalence of FcaGHV1 in Türkiye and to confirm the risk factors associated with FcaGHV1 detection.
期刊介绍：
Veterinary Medicine and Science is the peer-reviewed journal for rapid dissemination of research in all areas of veterinary medicine and science. The journal aims to serve the research community by providing a vehicle for authors wishing to publish interesting and high quality work in both fundamental and clinical veterinary medicine and science.
Veterinary Medicine and Science publishes original research articles, systematic reviews, meta-analyses, and research methods papers, along with invited editorials and commentaries. Original research papers must report well-conducted research with conclusions supported by the data presented in the paper.
We aim to be a truly global forum for high-quality research in veterinary medicine and science, and believe that the best research should be published and made widely accessible as quickly as possible. Veterinary Medicine and Science publishes papers submitted directly to the journal and those referred from a select group of prestigious journals published by Wiley-Blackwell.
Veterinary Medicine and Science is a Wiley Open Access journal, one of a new series of peer-reviewed titles publishing quality research with speed and efficiency. For further information visit the Wiley Open Access website.
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