Description of Porcibacter vitabionis gen. nov., sp. nov., an anaerobe isolated from pig faeces.

IF 2.3 3区生物学 Q4 MICROBIOLOGY

International journal of systematic and evolutionary microbiology Pub Date : 2026-07-01 DOI:10.1099/ijsem.0.007214

Jayoung Paek, Lu Bai, Jun-Pil Jang, Yeseul Shin, Hongik Kim, Si Hyun Kim, Jeong Hwan Shin, Joong-Ki Kook, Young-Hyo Chang

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引用次数: 0

Abstract

Two Gram-stain-positive rod-shaped anaerobic bacterial strains were isolated from pig faeces and designated as strains YH-ros2226^T and YH-ros2228. Phylogenetic analysis using 16S rRNA gene sequences revealed that the isolates were most closely related to Pararoseburia lenta KCTC 15957^T, with 92.2% similarity. The multi-locus sequence tree revealed that the isolates formed a distinct cluster within the family Lachnospiraceae. The average nucleotide identity, digital DNA-DNA hybridization, average amino acid identity and percentage of conserved proteins values between the isolates and related strains within the family Lachnospiraceae ranged from 67.0% to 68.8%, 18.5% to 33.9%, 51.5% to 54.6% and 33.6% to 44.1%, respectively. The major cellular fatty acids were C_16 : 0, C_18 : 1 ω9c and C_16 : 0 N alcohol. The cell wall peptidoglycan contained meso-diaminopimelic acid. The genomic DNA G+C contents of the strains were 46.8-47.0 mol%. The chemotaxonomic, phenotypic and phylogenetic properties of YH-ros2226^T (=KCTC 25944^T=JCM 37835^T) and YH-ros2228 (=KCTC 25945=JCM 37836) suggested that they represented a novel genus and species within the family Lachnospiraceae, for which the name Porcibacter vitabionis gen. nov., sp. nov. is proposed.

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从猪粪便中分离出的一种厌氧菌——活菌Porcibacter vitabionis gen. nov., sp. nov.。

从猪粪便中分离到2株革兰氏染色阳性棒状厌氧菌，分别命名为YH-ros2226T和YH-ros2228。采用16S rRNA基因序列进行系统发育分析，分离株与lenta Pararoseburia KCTC 15957T亲缘关系最近，相似度为92.2%。多位点序列树显示，分离物在毛缕菌科中形成一个明显的聚类。分离菌株与毛缕菌科亲缘菌株的核苷酸同源性、数字DNA-DNA杂交、氨基酸同源性和保守蛋白值百分比分别为67.0% ~ 68.8%、18.5% ~ 33.9%、51.5% ~ 54.6%和33.6% ~ 44.1%。主要的细胞脂肪酸是C16: 0、C18: 1 ω9c和C16: 0 N醇。细胞壁肽聚糖含有中二氨基亚苯甲酸。菌株基因组DNA G+C含量为46.8 ~ 47.0 mol%。YH-ros2226T （=KCTC 25944T=JCM 37835T）和YH-ros2228 （=KCTC 25945=JCM 37836）的化学分类、表型和系统发育特征表明，它们代表了毛螺科的一个新属和新种，并建议将其命名为Porcibacter vitabionis gen. nov., sp. nov.。

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来源期刊

International journal of systematic and evolutionary microbiology 生物-微生物学

CiteScore

5.20

自引率

21.40%

发文量

426

审稿时长

1 months

期刊介绍： Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.