An experimental study on the role of naloxone in facilitating alcohol metabolism in acute alcohol intoxication rats.

IF 2.5 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Future Science OA Pub Date : 2026-12-01 Epub Date: 2026-07-06 DOI:10.1080/20565623.2026.2698022

Ziqi Jin, Yingqi Hu, Xuqi Liu, Yingying Cao, Jiayi Zhang, Runa A, Xiaoxue Wang, Lingyu Zhang, Yongzhi Xue

{"title":"An experimental study on the role of naloxone in facilitating alcohol metabolism in acute alcohol intoxication rats.","authors":"Ziqi Jin, Yingqi Hu, Xuqi Liu, Yingying Cao, Jiayi Zhang, Runa A, Xiaoxue Wang, Lingyu Zhang, Yongzhi Xue","doi":"10.1080/20565623.2026.2698022","DOIUrl":null,"url":null,"abstract":"Objectives: Naloxone has been used in acute alcohol intoxication (AAI) in China and some other Asian countries. However, the effects of naloxone on alcohol metabolism and its mechanisms are unclear. This study aimed to explore the role of naloxone in facilitating alcohol metabolism.Methods: AAI rat model was established by force-feeding alcohol. The effect of naloxone in facilitating alcohol metabolism was detected using an improved breathalyzer. The open field test (OFT) was also used to observe the behavioral changes in rats.Results: By breathalyzer, naloxone could promote alcohol metabolism in a dose-dependent manner. Naloxone could promote the arousal of rats and increase the spontaneous activity of rats. But Cytochrome P450 2E1 (CYP2E1) protein and mRNA showed no significant changes. Naloxone promotes alcohol metabolism, and the changing trend of blood alcohol concentration shows a good pharmacokinetics-pharmacodynamics (PK-PD) correlation with adrenocorticotropic hormone (ACTH) expression and behavioral changes in rats.Conclusion: We established a method via gas collection bottles to evaluate the metabolic activity of CYP2E1 and alcohol dehydrogenase (ADH) in rats and established a metabolic model reflecting the changes in segmented alcohol concentrations over time. Naloxone could promote alcohol metabolism by promoting arousal, which is beneficial to AAI.","PeriodicalId":12568,"journal":{"name":"Future Science OA","volume":"12 1","pages":"2698022"},"PeriodicalIF":2.5000,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13348912/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Future Science OA","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/20565623.2026.2698022","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2026/7/6 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}

引用次数: 0

Abstract

Objectives: Naloxone has been used in acute alcohol intoxication (AAI) in China and some other Asian countries. However, the effects of naloxone on alcohol metabolism and its mechanisms are unclear. This study aimed to explore the role of naloxone in facilitating alcohol metabolism.

Methods: AAI rat model was established by force-feeding alcohol. The effect of naloxone in facilitating alcohol metabolism was detected using an improved breathalyzer. The open field test (OFT) was also used to observe the behavioral changes in rats.

Results: By breathalyzer, naloxone could promote alcohol metabolism in a dose-dependent manner. Naloxone could promote the arousal of rats and increase the spontaneous activity of rats. But Cytochrome P450 2E1 (CYP2E1) protein and mRNA showed no significant changes. Naloxone promotes alcohol metabolism, and the changing trend of blood alcohol concentration shows a good pharmacokinetics-pharmacodynamics (PK-PD) correlation with adrenocorticotropic hormone (ACTH) expression and behavioral changes in rats.

Conclusion: We established a method via gas collection bottles to evaluate the metabolic activity of CYP2E1 and alcohol dehydrogenase (ADH) in rats and established a metabolic model reflecting the changes in segmented alcohol concentrations over time. Naloxone could promote alcohol metabolism by promoting arousal, which is beneficial to AAI.

查看原文本刊更多论文

纳洛酮促进急性酒精中毒大鼠酒精代谢的实验研究。

目的：纳洛酮在中国和其他一些亚洲国家用于急性酒精中毒（AAI）。然而，纳洛酮对酒精代谢的影响及其机制尚不清楚。本研究旨在探讨纳洛酮在促进酒精代谢中的作用。方法：采用灌胃酒精法建立AAI大鼠模型。采用改进的呼气测醉仪检测纳洛酮促进酒精代谢的作用。采用开阔场试验（open field test， OFT）观察大鼠行为变化。结果：纳洛酮能促进酒精代谢，且呈剂量依赖性。纳洛酮能促进大鼠的觉醒，增加大鼠的自发活动。细胞色素P450 2E1 （CYP2E1）蛋白和mRNA无明显变化。纳洛酮促进酒精代谢，大鼠血液酒精浓度变化趋势与促肾上腺皮质激素（ACTH）表达及行为改变具有良好的药代动力学-药效学（PK-PD）相关性。结论：建立了用气体收集瓶法评价大鼠体内CYP2E1和乙醇脱氢酶（ADH）代谢活性的方法，建立了反映分段酒精浓度随时间变化的代谢模型。纳洛酮可通过促进觉醒来促进酒精代谢，对AAI有益。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Future Science OA MEDICINE, RESEARCH & EXPERIMENTAL-

CiteScore

5.00

自引率

4.00%

发文量

审稿时长

13 weeks

期刊介绍： Future Science OA is an online, open access, peer-reviewed title from the Future Science Group. The journal covers research and discussion related to advances in biotechnology, medicine and health. The journal embraces the importance of publishing all good-quality research with the potential to further the progress of research in these fields. All original research articles will be considered that are within the journal''s scope, and have been conducted with scientific rigour and research integrity. The journal also features review articles, editorials and perspectives, providing readers with a leading source of commentary and analysis. Submissions of the following article types will be considered: -Research articles -Preliminary communications -Short communications -Methodologies -Trial design articles -Trial results (including early-phase and negative studies) -Reviews -Perspectives -Commentaries