The Effect of Genetics on Caries Risk Assessment: MMP3 (rs679620) and VDR (rs731236) Gene Polymorphisms.

IF 1.7 4区医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Oral health & preventive dentistry Pub Date : 2026-07-03 DOI:10.3290/j.ohpd.c_2755

Seda Selvi, Pınar Yılmaz Atalı, Ömer Birkan Ağralı, Beste Tacal Aslan, Özlem Özge Yılmaz, Tolga Polat, Korkut Ulucan

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引用次数: 0

Abstract

Purpose: This study evaluated the impact of gene polymorphisms in VDR and MMP3 on caries and the efficacy of genetic factors in a novel caries risk model.

Methods and materials: After oral examination of male individuals (aged 20-44), a diagnosis was made according to the decayed, missing and filled teeth (DMFT) index: Group 1 'high caries risk' (DMFT ≥ 14); Group 2 'low caries risk' (DMFT ≤ 5) (n = 162). Participants' detailed anamneses were recorded. Periodontal indices, saliva buffering capacity/BC, salivary quantity of Streptococcus mutans/SM and Lactobacillus spp./LB were measured. After DNA isolation, genotyping of the MMP3 (rs679620) and VDR (rs731236) genes was conducted using real-time polymerase chain reaction (PCR). A stepwise regression analysis was conducted to evaluate the explanatory contributions of independent variables to DMFT variability. The results were considered significant at p 0.05.

Results: Significant differences were observed between the groups in terms of the plaque index, gingival index, probing depth, bleeding on probing, salivary flow rate, BC, salivary quantity of LB, frequency of consuming sugary foods/drinks, frequency of tooth brushing, and the use of preventive dental treatment (p 0.001). The MMP3 rs679620 polymorphism significantly affected caries risk (p 0.001), while the VDR polymorphism was not significant (p = 0.862). An explanatory regression model for caries experience, created by stepwise regression analysis, explained 61.1% of the DMFT.

Conclusion: Adding genetic factors to the caries risk model may have a significant effect on the accuracy of the model.

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遗传对龋病风险评估的影响：MMP3 （rs679620）和VDR （rs731236）基因多态性

目的：在一种新的龋病风险模型中，评估VDR和MMP3基因多态性对龋病的影响以及遗传因素的作用。方法与材料：对20 ~ 44岁男性进行口腔检查后，根据龋缺补牙（DMFT）指数进行诊断：1组“高龋风险”（DMFT≥14）；第二组为低龋风险组（DMFT≤5）（n = 162）。参与者的详细记忆被记录下来。测定牙周指标、唾液缓冲能力/BC、唾液变形链球菌数量/SM、乳酸杆菌数量/LB。DNA分离后，采用实时聚合酶链反应（PCR）对MMP3 （rs679620）和VDR （rs731236）基因进行分型。采用逐步回归分析评估自变量对DMFT变异的解释贡献。p < 0.05认为结果显著。结果：两组在牙菌斑指数、牙龈指数、探诊深度、探诊时出血、唾液流量、BC、唾液LB量、食用含糖食物/饮料的频率、刷牙频率、预防性牙科治疗的使用等方面均有显著差异（p < 0.001）。MMP3 rs679620多态性显著影响龋病风险（p = 0.001），而VDR多态性无显著影响（p = 0.862）。通过逐步回归分析建立龋病经历的解释性回归模型，对龋病的解释率为61.1%。结论：在龋病风险模型中加入遗传因素可能对模型的准确性有显著影响。

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来源期刊

Oral health & preventive dentistry DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.00

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Clinicians, general practitioners, teachers, researchers, and public health administrators will find this journal an indispensable source of essential, timely information about scientific progress in the fields of oral health and the prevention of caries, periodontal diseases, oral mucosal diseases, and dental trauma. Central topics, including oral hygiene, oral epidemiology, oral health promotion, and public health issues, are covered in peer-reviewed articles such as clinical and basic science research reports; reviews; invited focus articles, commentaries, and guest editorials; and symposium, workshop, and conference proceedings.