{"title":"Analytical comparison of two commercially available feline serum amyloid A assays using residual clinical plasma samples.","authors":"Ririna Shiba, Chie Tanaka, Rie Tsubouchi, Masaaki Ochi, Noboru Fujitani, Akihisa Hata","doi":"10.1292/jvms.26-0078","DOIUrl":null,"url":null,"abstract":"<p><p>Serum amyloid A (SAA) is a major acute-phase protein in cats; however, the agreement between commercially available feline SAA assays has not been fully evaluated. This study compared two analytical systems: the FUJI DRI-CHEM vf-SAA (sandwich fluorescence immunoassay) and VET-SAA Eiken (immunoturbidimetry). Eighty residual plasma samples from client-owned cats were analyzed. Methods were compared using Spearman's correlation, Passing-Bablok regression, and Bland-Altman percentage difference analysis. These assays revealed a strong association (Spearman's ρ=0.948). Passing-Bablok regression indicated proportional and constant bias (y=0.3325x + 1.155), and Bland-Altman analysis demonstrated a large negative mean percent difference (-86.2%). These findings suggest that the results are not interchangeable and method-specific reference intervals may be required.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"1314-1317"},"PeriodicalIF":1.1000,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13612957/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Veterinary Medical Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1292/jvms.26-0078","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2026/6/30 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Serum amyloid A (SAA) is a major acute-phase protein in cats; however, the agreement between commercially available feline SAA assays has not been fully evaluated. This study compared two analytical systems: the FUJI DRI-CHEM vf-SAA (sandwich fluorescence immunoassay) and VET-SAA Eiken (immunoturbidimetry). Eighty residual plasma samples from client-owned cats were analyzed. Methods were compared using Spearman's correlation, Passing-Bablok regression, and Bland-Altman percentage difference analysis. These assays revealed a strong association (Spearman's ρ=0.948). Passing-Bablok regression indicated proportional and constant bias (y=0.3325x + 1.155), and Bland-Altman analysis demonstrated a large negative mean percent difference (-86.2%). These findings suggest that the results are not interchangeable and method-specific reference intervals may be required.
期刊介绍：
JVMS is a peer-reviewed journal and publishes a variety of papers on veterinary science from basic research to applied science and clinical research. JVMS is published monthly and consists of twelve issues per year. Papers are from the areas of anatomy, physiology, pharmacology, toxicology, pathology, immunology, microbiology, virology, parasitology, internal medicine, surgery, clinical pathology, theriogenology, avian disease, public health, ethology, and laboratory animal science. Although JVMS has played a role in publishing the scientific achievements of Japanese researchers and clinicians for many years, it now also accepts papers submitted from all over the world.
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