{"title":"Novel HPLC-laser-stimulated fluorescence profiling of seminal plasma amino acids linked to ejaculate quality.","authors":"Megha Naik, Huidrom Yaiphaba Meitei, Shubhashree Uppangala, Dhakshanya Predheepan, Satish Kumar Adiga, Ajeetkumar Patil","doi":"10.1007/s10815-026-03944-9","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>Amino acids are vital biomolecules that can provide important insights into sperm functional competence. However, the level of amino acids (AAs) in seminal plasma and their implications on sperm characteristics remain less understood.</p><p><strong>Methods: </strong>For the first time, high-performance liquid chromatography (HPLC) in combination with a laser-stimulated fluorescence (LSF) system was used to profile AAs from the seminal plasma of 30 normozoospermic and 51 nonnormozoospermic ejaculates. Sperm characteristics, along with DNA integrity, were assessed and correlated with individual AAs.</p><p><strong>Results: </strong>The HPLC-LSF system was able to detect a total of 18 AAs in the seminal plasma. A significant variation was observed between the two groups for all AAs (p < 0.05), except for aspartate. Notably, the relative intensities of AAs were significantly lower in the non-normozoospermic group. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) further highlighted distinct clusters based on the AA profiles of the two groups. The pathway analysis indicated differences in the metabolic pathways between the normozoospermic and nonnormozoospermic groups.</p><p><strong>Conclusion: </strong>The HPLC-LSF platform could serve as a potential tool in identifying seminal plasma AA levels. The low relative abundance of AA in the non-normozoospermic men could be attributed to aberrant metabolic pathways, underscoring their significance in male infertility.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"2543-2555"},"PeriodicalIF":2.8000,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598000/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Assisted Reproduction and Genetics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s10815-026-03944-9","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2026/7/2 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 0
Abstract
Purpose: Amino acids are vital biomolecules that can provide important insights into sperm functional competence. However, the level of amino acids (AAs) in seminal plasma and their implications on sperm characteristics remain less understood.
Methods: For the first time, high-performance liquid chromatography (HPLC) in combination with a laser-stimulated fluorescence (LSF) system was used to profile AAs from the seminal plasma of 30 normozoospermic and 51 nonnormozoospermic ejaculates. Sperm characteristics, along with DNA integrity, were assessed and correlated with individual AAs.
Results: The HPLC-LSF system was able to detect a total of 18 AAs in the seminal plasma. A significant variation was observed between the two groups for all AAs (p < 0.05), except for aspartate. Notably, the relative intensities of AAs were significantly lower in the non-normozoospermic group. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) further highlighted distinct clusters based on the AA profiles of the two groups. The pathway analysis indicated differences in the metabolic pathways between the normozoospermic and nonnormozoospermic groups.
Conclusion: The HPLC-LSF platform could serve as a potential tool in identifying seminal plasma AA levels. The low relative abundance of AA in the non-normozoospermic men could be attributed to aberrant metabolic pathways, underscoring their significance in male infertility.
期刊介绍：
The Journal of Assisted Reproduction and Genetics publishes cellular, molecular, genetic, and epigenetic discoveries advancing our understanding of the biology and underlying mechanisms from gametogenesis to offspring health. Special emphasis is placed on the practice and evolution of assisted reproduction technologies (ARTs) with reference to the diagnosis and management of diseases affecting fertility. Our goal is to educate our readership in the translation of basic and clinical discoveries made from human or relevant animal models to the safe and efficacious practice of human ARTs. The scientific rigor and ethical standards embraced by the JARG editorial team ensures a broad international base of expertise guiding the marriage of contemporary clinical research paradigms with basic science discovery. JARG publishes original papers, minireviews, case reports, and opinion pieces often combined into special topic issues that will educate clinicians and scientists with interests in the mechanisms of human development that bear on the treatment of infertility and emerging innovations in human ARTs. The guiding principles of male and female reproductive health impacting pre- and post-conceptional viability and developmental potential are emphasized within the purview of human reproductive health in current and future generations of our species.
The journal is published in cooperation with the American Society for Reproductive Medicine, an organization of more than 8,000 physicians, researchers, nurses, technicians and other professionals dedicated to advancing knowledge and expertise in reproductive biology.
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