A Novel Endoscopic Biopsy Technique for Translational Research in Patients With Inflammatory Bowel Disease.

IF 3.6 3区医学 Q2 GASTROENTEROLOGY & HEPATOLOGY

Clinical and Translational Gastroenterology Pub Date : 2026-09-01 Epub Date: 2026-07-01 DOI:10.14309/ctg.0000000000001069

S van Zon, L Oldenburg, K Nachataya, R Goetgebuer, N de Boer, J Bernink, J Grootjans, G D'Haens

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引用次数: 0

Abstract

Introduction: Standard forceps biopsies often provide insufficient cellular yield for single-cell and spatial transcriptomic analyses. We assessed the feasibility of cold-snare resection in inflammatory bowel disease (IBD) and compared immune cell composition with standard forceps biopsies.

Methods: Patients with active IBD at endoscopy were included. One cold-snare resection and 2 standard biopsies were obtained from inflamed mucosa in the ileum, colon, or rectum. Sample quality and cell yield were evaluated using histology staining and flow cytometry. Adverse events (AEs) were recorded periprocedurally, at a 7-day follow-up, and up to 30 days.

Results: Thirty-four patients with IBD were included. Cold-snare resection was successful in all cases and yielded significantly higher number of T-cells (median 71 × 10 3 vs 17 × 10 3 , P = 0.016) and rare innate lymphoid cells (median 576 vs 127, P = 0.016) compared with standard forceps biopsies. Four mild AEs were reported.

Discussion: Cold-snare resection improves mucosal tissue integrity and cellular quality compared with standard biopsies, supporting its use for translational studies in IBD.

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一种用于炎症性肠病患者转化研究的新型内镜活检技术。

标准钳活检通常不能提供单细胞和空间转录组分析的细胞产率。我们评估了炎症性肠病（IBD）冷圈套切除术的可行性，并将免疫细胞组成与标准钳活检进行了比较。方法：纳入内镜下活动性IBD患者。从回肠、结肠或直肠的炎症粘膜进行一次冷阱切除术和两次标准活检。采用组织学染色和流式细胞术评价样品质量和细胞产量。不良事件（ae）记录围手术期，随访7天，最长30天。结果：纳入34例IBD患者。冷圈套切除术在所有病例中都是成功的，与标准钳活检相比，t细胞（中位数71×103 vs. 17×103, p=0.016）和罕见的先天淋巴样细胞（中位数576 vs. 127, p=0.016）的数量显著增加。报告轻度ae 4例。结论：与标准活检相比，冷阱切除术改善了粘膜组织完整性和细胞质量，支持其用于IBD的转化研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical and Translational Gastroenterology GASTROENTEROLOGY & HEPATOLOGY-

CiteScore

7.00

自引率

0.00%

发文量

114

审稿时长

16 weeks

期刊介绍： Clinical and Translational Gastroenterology (CTG), published on behalf of the American College of Gastroenterology (ACG), is a peer-reviewed open access online journal dedicated to innovative clinical work in the field of gastroenterology and hepatology. CTG hopes to fulfill an unmet need for clinicians and scientists by welcoming novel cohort studies, early-phase clinical trials, qualitative and quantitative epidemiologic research, hypothesis-generating research, studies of novel mechanisms and methodologies including public health interventions, and integration of approaches across organs and disciplines. CTG also welcomes hypothesis-generating small studies, methods papers, and translational research with clear applications to human physiology or disease. Colon and small bowel Endoscopy and novel diagnostics Esophagus Functional GI disorders Immunology of the GI tract Microbiology of the GI tract Inflammatory bowel disease Pancreas and biliary tract Liver Pathology Pediatrics Preventative medicine Nutrition/obesity Stomach.