MSC-derived extracellular vesicles accelerate wound healing in senescent fibroblast cultures.

IF 5.3 4区医学 Q1 GERIATRICS & GERONTOLOGY

Biogerontology Pub Date : 2026-07-02 DOI:10.1007/s10522-026-10468-3

Ekaterina Rudnitsky, Naomy Vineshtock, Natali Yakubov, Alex Braiman, Yael Segev, Marina Wolfson, Khachik K Muradian, Vera Gorbunova, Gadi Turgeman, Michaela Ben Shahar, Vadim E Fraifeld

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Abstract

Wound healing (WH) is a multi-component and highly orchestrated process, in which fibroblasts play a pivotal role. Wound healing is commonly attenuated during aging and the course of cellular senescence (CS). A growing body of evidence demonstrates that mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) can mitigate the aging and CS phenotype. In this study, we evaluated the effects of EVs derived from naïve MSCs (nMSCs) or anti-inflammatory polarized MSCs (pMSCs) on the rate of in vitro wound healing in primary cultures of human pulmonary fibroblasts (HPF) at various stages of cellular senescence. The major finding of this study is that MSC-derived EVs increase the rate of in vitro WH in senescent but not young HPF cultures. Yet, the pMSC-derived EVs had a stronger impact on WH acceleration in senescent cultures as compared to the nMSC-derived EVs, and this impact was apparently attributed to cell migration.

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间充质干细胞衍生的细胞外囊泡加速衰老成纤维细胞培养的伤口愈合。

伤口愈合（WH）是一个多组分和高度协调的过程，其中成纤维细胞起关键作用。伤口愈合通常在衰老和细胞衰老（CS）过程中减弱。越来越多的证据表明，间充质干细胞（MSC）衍生的细胞外囊泡（EVs）可以减缓衰老和CS表型。在这项研究中，我们评估了naïve间充质干细胞（nMSCs）或抗炎极化间充质干细胞（pMSCs）衍生的ev对处于细胞衰老不同阶段的人肺成纤维细胞（HPF）原代培养的体外伤口愈合速度的影响。本研究的主要发现是，msc衍生的ev增加了老年HPF培养中体外WH的比率，而不是年轻HPF培养。然而，与nmsc衍生的电动汽车相比，pmsc衍生的电动汽车对衰老培养物中WH加速的影响更大，这种影响显然归因于细胞迁移。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biogerontology 医学-老年医学

CiteScore

8.00

自引率

4.40%

发文量

审稿时长

>12 weeks

期刊介绍： The journal Biogerontology offers a platform for research which aims primarily at achieving healthy old age accompanied by improved longevity. The focus is on efforts to understand, prevent, cure or minimize age-related impairments. Biogerontology provides a peer-reviewed forum for publishing original research data, new ideas and discussions on modulating the aging process by physical, chemical and biological means, including transgenic and knockout organisms; cell culture systems to develop new approaches and health care products for maintaining or recovering the lost biochemical functions; immunology, autoimmunity and infection in aging; vertebrates, invertebrates, micro-organisms and plants for experimental studies on genetic determinants of aging and longevity; biodemography and theoretical models linking aging and survival kinetics.