Myc sustains sex-biased organ zonation in the Drosophila intestine.

IF 9.2 1区生物学 Q1 CELL BIOLOGY

Developmental cell Pub Date : 2026-07-08 Epub Date: 2026-06-30 DOI:10.1016/j.devcel.2026.06.003

Christopher Amourda, Laura Blackie, Pedro Gaspar, Laura Martin-Coll, Anadika R Prasad, Andrew H House, Minna Holopainen, Hanna Ruhanen, Nicole Lamichane, Dafni Hadjieconomou, Tomotsune Ameku, Alessandro Mineo, Milly Coxhead, Irene Soler-Sáez, Cristina Galiana-Roselló, Francisco García-Garcia, Yi-Fang Wang, Cyrille Alexandre, Ville Hietakangas, Reijo Käkelä, Santiago Vernia, Irene Miguel-Aliaga

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引用次数: 0

Abstract

Organs exhibit distinct transcriptional and metabolic territories; how and why such organ zonation differs between the sexes is unclear. Using adult Drosophila intestines, we combine whole-organ 3D cellular mapping with metabolic profiling to uncover regional differences in gene expression and metabolism that vary by sex and genotype. Zonation emerges from opposing, region-specific, and sex-biased actions of the sex determinant doublesex (Dsx) and the transcription factor Myc. In enterocytes, male-specific Dsx represses Myc, resulting in female-biased Myc expression. Myc acts in specific gut regions to increase enterocyte ploidy in females. Myc also sustains a female-specific lipid profile, marked by sex-biased expression of a cluster of lipases and selective triacylglycerol storage in the anterior midgut. Myc activity in female enterocytes sustains peripheral lipid reserves and fecundity. These findings identify relevant roles for Myc in sex differentiation and the intestine as a key, previously overlooked contributor to sex differences in lipid metabolism.

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Myc在果蝇肠道中维持性别偏向的器官区分。

器官表现出不同的转录和代谢领域；这种器官分区在两性之间是如何以及为什么不同的还不清楚。利用成年果蝇的肠道，我们将全器官3D细胞图谱与代谢谱相结合，揭示基因表达和代谢的区域差异，这些差异因性别和基因型而异。分区产生于性别决定因子双性（Dsx）和转录因子Myc的对立、区域特异性和性别偏向行为。在肠细胞中，男性特异性的Dsx抑制Myc，导致女性偏向的Myc表达。Myc作用于特定的肠道区域，增加雌性肠细胞倍性。Myc还维持着女性特有的脂质特征，其特征是一组脂肪酶的性别偏倚表达和中肠前部的选择性甘油三酯储存。Myc活性在雌性肠细胞维持外周脂质储备和繁殖力。这些发现确定了Myc在性别分化中的相关作用，以及肠道在脂质代谢的性别差异中是一个关键的、以前被忽视的因素。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Developmental cell 生物-发育生物学

CiteScore

18.90

自引率

1.70%

发文量

203

审稿时长

3-6 weeks

期刊介绍： Developmental Cell, established in 2001, is a comprehensive journal that explores a wide range of topics in cell and developmental biology. Our publication encompasses work across various disciplines within biology, with a particular emphasis on investigating the intersections between cell biology, developmental biology, and other related fields. Our primary objective is to present research conducted through a cell biological perspective, addressing the essential mechanisms governing cell function, cellular interactions, and responses to the environment. Moreover, we focus on understanding the collective behavior of cells, culminating in the formation of tissues, organs, and whole organisms, while also investigating the consequences of any malfunctions in these intricate processes.