M. Bobkova, P. Filatov, P. Luganskaya, G. Bobkov, D. Gorbenko
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引用次数: 0
Abstract
A local surface plasmon resonance (LSPR)-based biosensor utilizing four-way junction (4WJ) DNA probes and gold nanoparticles (Au NPs) was developed for detection and genotyping of Mycobacterium tuberculosis. The sensor combines Au nanorods functionalized with a 4WJ probe and universal molecular beacon (UMB)-capped Au nanospheres, enabling target-induced nanoparticle assembly only in a presence of specific DNA analyte. These sensors achieved a limit of detection (LOD) of 8.5 nM for rifampicin-resistant M. tuberculosis DNA for fluorescent detection and 50 nM for LSPR detection, demonstrating specificity for single-nucleotide variation (SNV) discrimination in the vapC37 gene. The approach offers a new cost-effective, rapid combination of 2 technologies: conventional SPR microscopy and 4WJ probes, with potential post-amplification applications in point-of-care tuberculosis diagnostics.
期刊介绍：
Doklady Physical Chemistry is a monthly journal containing English translations of current Russian research in physical chemistry from the Physical Chemistry sections of the Doklady Akademii Nauk (Proceedings of the Russian Academy of Sciences). The journal publishes the most significant new research in physical chemistry being done in Russia, thus ensuring its scientific priority. Doklady Physical Chemistry presents short preliminary accounts of the application of the state-of-the-art physical chemistry ideas and methods to the study of organic and inorganic compounds and macromolecules; polymeric, inorganic and composite materials as well as corresponding processes. The journal is intended for scientists in all fields of chemistry and in interdisciplinary sciences.
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