Enhancing the conception rate in water buffalo using post-AI intravaginal bio-stimulation and clitoral massage.

IF 1.6 Q3 VETERINARY SCIENCES

Open Veterinary Journal Pub Date : 2026-05-01 Epub Date: 2026-05-31 DOI:10.5455/OVJ.2026.v16.i5.53

Anup Sarker, Dibyendu Biswas, Ashit Kumar Paul

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引用次数: 0

Abstract

Background: Suboptimal reproductive performance following artificial insemination (AI) remains a primary constraint to the profitability of buffalo farming in Bangladesh.

Aim: This study aimed to evaluate the efficacy of intravaginal bio-stimulation and clitoral massage (CM) in enhancing the post-AI conception rates in buffaloes.

Methods: A total of 125 female buffaloes were randomly selected, and their age, parity, breed, body condition score, reproductive health (RH), and calving difficulty were recorded. The buffaloes were divided into five groups (n = 25 in each group): group A (natural mating), group B (AI during observed estrus), group C [AI followed by penis-like device (PLD) application], group D (AI with CM), and group E (AI followed by PLD and CM). Group B (only AI) served as the control for the intervention groups (C, D, and E).

Results: The overall conception rate was 46.4%, with group A having the highest rate (64.0%). The conception rate of group E (52.0%) was higher than that of groups C (44.0%), D (40.0%), and B (32.0%). Parity and previous calving difficulty significantly influenced the conception rates (p < 0.05). Buffaloes aged between 4.6 and 5.5 years were 4.3 times more likely to conceive compared with other age groups (p < 0.05). Correlation coefficient analysis revealed a significant positive correlation between age and parity (p < 0.01) and a significant negative correlation with the RH of cows.

Conclusion: Intravaginal bio-stimulation using a PLD combined with CM post-AI significantly improves the conception rate in buffaloes.

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人工授精后阴道内生物刺激和阴蒂按摩提高水牛受孕率。

背景：人工授精（AI）后的次优生殖性能仍然是孟加拉国水牛养殖盈利能力的主要制约因素。目的：本研究旨在评价阴道内生物刺激和阴蒂按摩（CM）对提高人工授精后水牛受孕率的作用。方法：随机选取125头雌性水牛，记录其年龄、胎次、品种、体况评分、生殖健康（RH）、产犊困难等指标。将水牛分为5组（每组25只）：A组（自然交配）、B组（观察发情期间人工授精）、C组（人工授精后施阴茎样装置）、D组（人工授精后施阴茎样装置）、E组（人工授精后施阴茎样装置和人工授精）。B组（仅AI）为干预组（C、D、E）的对照组。结果：总受孕率为46.4%，以A组最高，为64.0%。E组受孕率（52.0%）高于C组（44.0%）、D组（40.0%）和B组（32.0%）。胎次和产犊困难对受孕率有显著影响（p < 0.05）。4.6 ~ 5.5岁水牛的怀孕率是其他年龄组的4.3倍（p < 0.05）。相关系数分析显示，奶牛年龄与胎次呈极显著正相关（p < 0.01），与RH呈极显著负相关。结论：阴道内生物刺激联合体外受精可显著提高水牛受孕率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Open Veterinary Journal VETERINARY SCIENCES-

CiteScore

1.40

自引率

0.00%

发文量

112

审稿时长

12 weeks

期刊介绍： Open Veterinary Journal is a peer-reviewed international open access online and printed journal that publishes high-quality original research articles. reviews, short communications and case reports dedicated to all aspects of veterinary sciences and its related subjects. Research areas include the following: Infectious diseases of zoonotic/food-borne importance, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, epidemiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology, oncology and animal reproduction. All papers are peer-reviewed. Moreover, with the presence of well-qualified group of international referees, the process of publication will be done meticulously and to the highest standards.