Genotypic detection of some virulence factors and genetic tree of Staphylococcus aureus isolated from buffalo with subclinical mastitis in Nineveh governorate.
{"title":"Genotypic detection of some virulence factors and genetic tree of <i>Staphylococcus aureus</i> isolated from buffalo with subclinical mastitis in Nineveh governorate.","authors":"Fahad Yaseen Taha, Ayman Hani Taha, Omar Hashim Sheet","doi":"10.5455/OVJ.2026.v16.i5.59","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong><i>Staphylococcus aureus</i> (<i>S. aureus</i>) is identified as the foremost cause of inflammation of the milk-secreting glands of buffalo and all ruminant animals. This bacterium carries various genes that encode virulence factors.</p><p><strong>Aims: </strong>This study aimed to identify the prevalence of <i>S. aureus</i> in milk specimens from buffalo farms, identify specific genes associated with virulence, and compare the genetic similarity of the study isolates with <i>S. aureus</i> isolates from different regions worldwide.</p><p><strong>Methods: </strong>Sixty subclinical milk samples were collected from buffaloes from October to December 2024 across various locations in the Nineveh governorate. This study employed standard microbiological procedures, including the use of various types of media and biochemical tests. Additionally, the presence of <i>nuc, mecA, clfA, clfB, sea, seb</i>, and <i>sec</i> genes in the isolates was determined using polymerase chain reaction.</p><p><strong>Results: </strong><i>Staphylococcus aureus</i> was detected in 15% (9/60) of buffalo subclinical mastitis milk samples, with a higher prevalence rate of 30% (3/10) in buffalo milk samples from the Kaneitra district. While <i>nuc</i>, <i>clfA</i>, and <i>clfB</i> were present in all <i>S. aureus</i> isolates, the <i>mecA</i>, <i>sea</i>, <i>seb</i>, and <i>sec</i> genes were not. Using <i>clfA</i> gene analysis, five distinct strain sequences were identified and deposited to GenBank.</p><p><strong>Conclusions: </strong>Five <i>S. aureus</i> strains were found to share characteristics with strains from all over the world using phylogenetic analysis; the isolates exhibit distinct characteristics according to strain type and global distribution.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":"16 5","pages":"3192-3202"},"PeriodicalIF":1.6000,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13314351/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Open Veterinary Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5455/OVJ.2026.v16.i5.59","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2026/5/31 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Staphylococcus aureus (S. aureus) is identified as the foremost cause of inflammation of the milk-secreting glands of buffalo and all ruminant animals. This bacterium carries various genes that encode virulence factors.
Aims: This study aimed to identify the prevalence of S. aureus in milk specimens from buffalo farms, identify specific genes associated with virulence, and compare the genetic similarity of the study isolates with S. aureus isolates from different regions worldwide.
Methods: Sixty subclinical milk samples were collected from buffaloes from October to December 2024 across various locations in the Nineveh governorate. This study employed standard microbiological procedures, including the use of various types of media and biochemical tests. Additionally, the presence of nuc, mecA, clfA, clfB, sea, seb, and sec genes in the isolates was determined using polymerase chain reaction.
Results: Staphylococcus aureus was detected in 15% (9/60) of buffalo subclinical mastitis milk samples, with a higher prevalence rate of 30% (3/10) in buffalo milk samples from the Kaneitra district. While nuc, clfA, and clfB were present in all S. aureus isolates, the mecA, sea, seb, and sec genes were not. Using clfA gene analysis, five distinct strain sequences were identified and deposited to GenBank.
Conclusions: Five S. aureus strains were found to share characteristics with strains from all over the world using phylogenetic analysis; the isolates exhibit distinct characteristics according to strain type and global distribution.
期刊介绍：
Open Veterinary Journal is a peer-reviewed international open access online and printed journal that publishes high-quality original research articles. reviews, short communications and case reports dedicated to all aspects of veterinary sciences and its related subjects. Research areas include the following: Infectious diseases of zoonotic/food-borne importance, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, epidemiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology, oncology and animal reproduction. All papers are peer-reviewed. Moreover, with the presence of well-qualified group of international referees, the process of publication will be done meticulously and to the highest standards.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
