Bioaccessibility of metals in food supplements: protocols and facts

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biometals Pub Date : 2026-06-30 DOI:10.1007/s10534-026-00844-4

Thaís dos Santos Berón, Gustavo Rossato Bitencourt, Rochele Sogari Picoloto, Rodrigo Cordeiro Bolzan, Paola Azevedo Mello

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引用次数: 0

Abstract

This study evaluated the bioaccessibility of essential (Ca, Cu, Fe, K, Mg, Mn, Mo, Na and Zn) and non-essential (Al, Ba, Cr, Sr and V) elements in food supplements (vitamins and/or minerals and botanicals). Extraction using in-vitro methods (Unified BARGE Method—UBM and INFOGEST) was evaluated allowing a comparison of protocols and using inductively coupled plasma optical emission spectrometry (ICP OES). Bioaccessibility values revealed differences among the elements in different types of food supplements. The limits of quantification (LOQs) obtained for some elements were relatively high for both methods due to the composition of the digests, which drastically impaired bioaccessibility assessment. As a result, most elements presented fractions lower than the LOQ, regardless of the sample. This is an important limitation promoted by interferences when analyzing these extracts by ICP-based techniques. A simple extraction using 1 mol L⁻¹ HCl was evaluated, considering that HCl is present in the solutions used in both protocols. Leaching was observed for the same elements using only the HCl solution.

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食品补充剂中金属的生物可及性：协议和事实。

本研究评估了食品补充剂（维生素和/或矿物质和植物药）中必需元素（Ca、Cu、Fe、K、Mg、Mn、Mo、Na和Zn）和非必需元素（Al、Ba、Cr、Sr和V）的生物可及性。采用体外提取方法（统一BARGE法- ubm和INFOGEST）进行评估，允许比较方案和使用电感耦合等离子体光学发射光谱法（ICP OES）。生物可及性值揭示了不同类型食品补充剂中元素之间的差异。由于消化酶的组成，两种方法对某些元素的定量限（loq）都相对较高，这严重影响了生物可及性评价。因此，无论样品如何，大多数元素的分数都低于定量限。当使用基于icp的技术分析这些提取物时，这是一个由干扰引起的重要限制。考虑到在两种方案中使用的溶液中都存在HCl，对使用1 mol L-1 HCl的简单提取进行了评估。对相同的元素，仅使用盐酸溶液就可以观察到浸出。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biometals 生物-生化与分子生物学

CiteScore

5.90

自引率

8.60%

发文量

111

审稿时长

3 months

期刊介绍： BioMetals is the only established journal to feature the important role of metal ions in chemistry, biology, biochemistry, environmental science, and medicine. BioMetals is an international, multidisciplinary journal singularly devoted to the rapid publication of the fundamental advances of both basic and applied research in this field. BioMetals offers a forum for innovative research and clinical results on the structure and function of: - metal ions - metal chelates, - siderophores, - metal-containing proteins - biominerals in all biosystems. - BioMetals rapidly publishes original articles and reviews. BioMetals is a journal for metals researchers who practice in medicine, biochemistry, pharmacology, toxicology, microbiology, cell biology, chemistry, and plant physiology who are based academic, industrial and government laboratories.