Alterations in antioxidant minerals and oxidative stress biomarkers with altitude among young Indian males

IF 3 3区地球科学 Q2 BIOPHYSICS

International Journal of Biometeorology Pub Date : 2026-06-30 DOI:10.1007/s00484-026-03257-7

Sneha Chopra, Vishwendra Vikram Singh, Vijay Kumar Singh, Saroj Kumar Verma, Shiva Saxena, Vandana Kirar, Pulkit Mathur, Som Nath Singh

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引用次数: 0

Abstract

Chronic exposure to hypoxia occurs in lowland populations, including soldiers, adventurers, tourists, and porters, during their ascent to high altitude. Hypobaric hypoxia induces oxidative stress and impairs the metabolism of micro minerals, including selenium, zinc, iron, and copper. The role of metalloproteins and their associated microminerals in preventing oxidative stress at different altitudes is incompletely understood. In this study, plasma samples were collected from lowlanders at sea level (SL), moderate altitude (A1-1585 m, A2-2290 m), and high altitude (A3-3500 m). Inductively Coupled Plasma Mass Spectrometry (ICP-MS) was used to quantify selenium (Se), zinc (Zn), copper (Cu), and iron (Fe) levels. Antioxidant metalloproteins, including Copper-Zinc Superoxide dismutase (Cu-Zn SOD), Glutathione Peroxidase (GPx), Metallothionein (MT), Selenoprotein 1 (SEPP1), were quantified by enzyme-linked immunosorbent assay. Lipid peroxidation was assessed through malondialdehyde (MDA) and hydroperoxides through ferrous oxidation xylenol orange; FOX1 assay. We found that Se, Zn, Cu, and Fe levels were higher (p < 0.01) at A2 compared to SL and lower (p < 0.01) at A3 compared to A2. MT levels were higher (p < 0.01) at A1 and A3 compared to SL. SEPP1 levels were higher (p < 0.001) at A3 compared to SL, A1, and A2. Cu-Zn SOD levels were higher at A3 (p = 0.006). GPx levels remained consistent across all altitudes. MDA levels were significantly elevated (p = 0.013) at A3 compared to SL, while the FOX assay showed increased hydroperoxide levels (p < 0.001) at A2 and A3 as compared to SL. Our findings demonstrate that hypoxia leads to variations in micromineral levels at different altitudes, accompanied by elevated oxidative stress and dysregulated antioxidant metalloproteins potentially reflecting adaptive responses to oxidative stress at high altitude.

Graphical abstract

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印度年轻男性抗氧化矿物质和氧化应激生物标志物随海拔的变化。

慢性缺氧发生在低地人群中，包括士兵、冒险家、游客和搬运工，在他们上升到高海拔的过程中。低气压缺氧诱导氧化应激，损害微量矿物质的代谢，包括硒、锌、铁和铜。金属蛋白及其相关微量元素在不同海拔地区预防氧化应激中的作用尚不完全清楚。在本研究中，血浆样本采集于低海拔地区（SL）、中海拔地区（A1-1585 m, A2-2290 m）和高海拔地区（A3-3500 m）。采用电感耦合等离子体质谱法（ICP-MS）定量测定硒（Se）、锌（Zn）、铜（Cu）和铁（Fe）水平。采用酶联免疫吸附法测定抗氧化金属蛋白，包括铜锌超氧化物歧化酶（Cu-Zn SOD）、谷胱甘肽过氧化物酶（GPx）、金属硫蛋白（MT）、硒蛋白1 （SEPP1）。脂质过氧化通过丙二醛（MDA）和氢过氧化物通过亚铁氧化二甲醇橙进行评估；FOX1化验。我们发现硒、锌、铜和铁的含量更高(p

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来源期刊

International Journal of Biometeorology 地学-环境科学

CiteScore

6.40

自引率

9.40%

发文量

183

审稿时长

1 months

期刊介绍： The Journal publishes original research papers, review articles and short communications on studies examining the interactions between living organisms and factors of the natural and artificial atmospheric environment. Living organisms extend from single cell organisms, to plants and animals, including humans. The atmospheric environment includes climate and weather, electromagnetic radiation, and chemical and biological pollutants. The journal embraces basic and applied research and practical aspects such as living conditions, agriculture, forestry, and health. The journal is published for the International Society of Biometeorology, and most membership categories include a subscription to the Journal.