The effect of voluntary chronic alcohol consumption on Th1/Th2 cytokine signature during adolescence in Sprague-Dawley Rats.

IF 2.2 Q2 MEDICINE, GENERAL & INTERNAL

SAGE Open Medicine Pub Date : 2026-06-18 eCollection Date: 2026-01-01 DOI:10.1177/20503121261434123

Kutlwano Moeketsi, Bongani B Nkambule, Kennedy H Erlwanger, Xitsakiso E Mabasa, Muzi J Maseko, Tawanda M Nyambuya

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Abstract

Background: Chronic alcohol use is a global health concern with adverse effects on immune function, potentially altering the T-helper (Th)1/Th2 axis. This study modelled chronic alcohol consumption using Sprague-Dawley (SD) rats to determine whether chronic alcohol consumption during adolescence skews Th1/Th2 cytokine release.

Methods: A two-bottle choice rat model was used for voluntary adolescent alcohol intake. Twenty (n = 20) male SD rats aged 25 days were randomised into alcohol (20% w/v) and control groups (n = 10/group) for 30 days. Blood was collected at termination via cardiac puncture. Th1/Th2 cytokines were analysed using the Rat LEGENDplex™ Panel (9-plex) kit and BD Accuri™ C6 flow cytometer. An unpaired two-tailed student's t-test compared the cytokine level between the groups.

Results: White blood cell and lymphocyte counts were elevated in the alcohol group, while platelet counts decreased (p < 0.05). Interleukin (IL)-2 (p = 0.0390) and interferon-γ (p = 0.0292) were lower in the alcohol group. Similarly, IL-5 (p = 0.0189), IL-6 (p = 0.0284) and IL-10 (p = 0.0117) levels decreased.

Conclusion: Chronic voluntary alcohol consumption during adolescence does not skew the immune response towards Th1 or Th2 cytokine release. Instead, it is associated with an overall decrease in the levels of Th1/Th2 cytokines, thus suggesting the immune-suppressive effect of alcohol.

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Sprague-Dawley大鼠青春期自愿慢性饮酒对Th1/Th2细胞因子特征的影响

背景：慢性饮酒是一个全球性的健康问题，对免疫功能有不良影响，可能改变辅助性t细胞（Th）1/Th2轴。本研究使用SD大鼠模拟慢性酒精消耗，以确定青春期慢性酒精消耗是否会扭曲Th1/Th2细胞因子的释放。方法：采用双瓶选择大鼠模型进行青少年酒精自愿摄入。25日龄雄性SD大鼠20只（n = 20），随机分为酒精（20% w/v）组和对照组（n = 10/组），试验期30 d。终止时通过心脏穿刺采血。使用大鼠LEGENDplex™Panel （9-plex）试剂盒和BD Accuri™C6流式细胞仪分析Th1/Th2细胞因子。一个未配对的双尾学生t检验比较了两组之间的细胞因子水平。结果：酒精组白细胞和淋巴细胞计数升高，血小板计数降低（p = 0.0390），干扰素-γ (p = 0.0292)降低。同样，IL-5 （p = 0.0189）、IL-6 （p = 0.0284）和IL-10 （p = 0.0117）水平下降。结论：青春期长期自愿饮酒不会扭曲免疫应答对Th1或Th2细胞因子释放的影响。相反，它与Th1/Th2细胞因子水平的总体下降有关，因此表明酒精具有免疫抑制作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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