In Vitro Effect of Lithium Chloride on Adipose Tissue Derived Stem Cells Proliferation and Growth Kinetic.

IF 1.6 Q2 MEDICINE, GENERAL & INTERNAL

Iranian Journal of Medical Sciences Pub Date : 2026-06-01 DOI:10.30476/ijms.2026.108735.4372

Mahin Homayoon, Davood Mehrabani, Mohammad Amin Edalatmanesh, Mehrdad Shariati, Masood Amini

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引用次数: 0

Abstract

Background: Adipose tissue-derived stem cells (AdSCs) are widely used for regenerative medicine purposes. There is always a need to accelerate cell proliferation and protect the cells from apoptosis when cell transplantation, especially AdSCs, is targeted. Therefore, this study evaluated the effect of lithium chloride on the proliferation and growth kinetic of AdSCs.

Methods: The study was undertaken in the Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran, in 2020. Adipose tissue specimens were provided from the abdominal region of a 35-year-old woman. In an in vitro study, human AdSCs were characterized morphologically, by osteo- and adipogenic differentiation properties, and by flow cytometry. They were later treated with lithium chloride to evaluate its effect on cell proliferation and the growth kinetics of AdSCs. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay investigated lithium chloride effect on the proliferation rate and apoptosis of AdSCs. Quantitative Real Time Polymerase Chain Reaction (qPCR) was carried out to determine the expression of apoptosis genes of Apoptosis Regulator BCL2 Associated X (BAX) and B-cell lymphoma 2 (Bcl-2) protein family.

Results: AdSCs showed mesenchymal characteristics, and lithium chloride at a dose of 6 µM had an increasing impact on cell proliferation and protected the cells from apoptosis. A non-significant increase in expression of the Bcl-2 gene (P=0.057) and a significant decrease in BAX gene (P=0.034) expression were visible.

Conclusion: Our findings indicate that the addition of lithium chloride to the culture medium enhanced the proliferation of AdSCs and protected them against apoptosis.

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氯化锂对脂肪组织源性干细胞增殖和生长动力学的体外影响。

背景：脂肪组织源性干细胞（AdSCs）被广泛应用于再生医学。细胞移植，尤其是AdSCs的移植，总是需要加速细胞增殖和保护细胞免于凋亡。因此，本研究评估氯化锂对AdSCs增殖和生长动力学的影响。方法：本研究于2020年在伊朗设拉子医学大学干细胞技术研究中心进行。脂肪组织标本来自一名35岁妇女的腹部。在一项体外研究中，通过成骨和脂肪分化特性以及流式细胞术对人AdSCs进行了形态学表征。然后用氯化锂处理它们，以评估其对细胞增殖和AdSCs生长动力学的影响。3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四唑（MTT）实验研究氯化锂对AdSCs增殖率和凋亡的影响。采用定量实时聚合酶链式反应（qPCR）检测凋亡调节因子BCL2 Associated X （BAX）和b细胞淋巴瘤2 （Bcl-2）蛋白家族凋亡基因的表达。结果：AdSCs具有间充质特性，6µM氯化锂对细胞增殖的影响增强，对细胞凋亡具有保护作用。Bcl-2基因表达无显著升高（P=0.057）， BAX基因表达显著降低（P=0.034）。结论：在培养基中添加氯化锂可促进AdSCs的增殖，并对其凋亡具有保护作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Medical Sciences MEDICINE, GENERAL & INTERNAL-

CiteScore

3.20

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Medical Sciences (IJMS) is an international quarterly biomedical publication, which is sponsored by Shiraz University of Medical Sciences. The IJMS intends to provide a scientific medium of communication for researchers throughout the globe. The journal welcomes original clinical articles as well as clinically oriented basic science research experiences on prevalent diseases in the region and analysis of various regional problems.