A new device for simultaneous cooling of the drop and evaporated sample for the extraction of volatile components using cooled side arm tube headspace microextraction.

IF 1.8 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of chromatographic science Pub Date : 2026-06-18 DOI:10.1093/chromsci/bmag020

Hamzeh Adelpour Abdoli, Kamal Alizadeh, Payman Hashemi

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引用次数: 0

Abstract

A new cooled side arm tube headspace single drop microextraction (CSAT-HS-SDME) method was successfully applied to the extraction of Carum copticum volatile components. The aim of this research is to design and fabricate a new device for simultaneous cooling of the drop and evaporated sample. The innovated technique was evaluated by the extraction and Gas chromatography-mass spectrometer (GC-MS) determination of p-cymene, γ-terpinene and thymol in C. copticum samples. Optimization of the effective extraction parameters was carried out by the simplex method. Under the optimized conditions (i.e. cold and heat temperature, -10°C and 70°C; water addition, 20 μL and extraction time, 20 min), the relative standard deviations of peak areas obtained from five replications were between 3.62% and 4.69%. Compared to other similar equipment for cooling the extraction phase and simultaneously heating the sample, this device has advantages such as simplicity, very low cost, ease of use, portability and no need for additional equipment such as a liquid carbon dioxide cylinder or heater-stirrer. The proposed method was successfully applied to the extraction and identification of volatile components of some C. copticum samples.

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一种同时冷却液滴和蒸发样品的新装置，用于利用冷却侧臂管顶空微萃取萃取挥发性成分。

采用一种新型冷却侧臂管顶空单滴微萃取（CSAT-HS-SDME）方法，成功地提取了黄连挥发物。本研究的目的是设计和制造一种同时冷却液滴和蒸发样品的新装置。通过提取和气相色谱-质谱联用（GC-MS）测定黄连样品中的对伞花素、γ-萜烯和百里香酚，对该技术进行了评价。采用单纯形法对提取工艺参数进行优化。在最佳条件下（冷热温度分别为-10℃和70℃，加水量为20 μL，提取时间为20 min）， 5次重复峰面积的相对标准偏差在3.62% ~ 4.69%之间。与其他用于冷却萃取相并同时加热样品的类似设备相比，该设备具有简单，成本极低，易于使用，便携性等优点，并且不需要额外的设备，如液体二氧化碳圆柱体或加热搅拌器。该方法成功地应用于部分黄连样品中挥发性成分的提取和鉴定。

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来源期刊

Journal of chromatographic science 化学-分析化学

CiteScore

2.90

自引率

7.70%

发文量

审稿时长

5.6 months

期刊介绍： The Journal of Chromatographic Science is devoted to the dissemination of information concerning all methods of chromatographic analysis. The standard manuscript is a description of recent original research that covers any or all phases of a specific separation problem, principle, or method. Manuscripts which have a high degree of novelty and fundamental significance to the field of separation science are particularly encouraged. It is expected the authors will clearly state in the Introduction how their method compares in some markedly new and improved way to previous published related methods. Analytical performance characteristics of new methods including sensitivity, tested limits of detection or quantification, accuracy, precision, and specificity should be provided. Manuscripts which describe a straightforward extension of a known analytical method or an application to a previously analyzed and/or uncomplicated sample matrix will not normally be reviewed favorably. Manuscripts in which mass spectrometry is the dominant analytical method and chromatography is of marked secondary importance may be declined.