Andres Garcia, Akash Garg, Feimo Yang, O. Burak Ozdoganlar, Philip R. LeDuc
{"title":"Fast Image Segmentation Toward Automation of 3D Ice Printing","authors":"Andres Garcia, Akash Garg, Feimo Yang, O. Burak Ozdoganlar, Philip R. LeDuc","doi":"10.1021/cbmi.5c00064","DOIUrl":null,"url":null,"abstract":"<p>Freeform 3D ice printing is emerging as a promising additive\r\nmanufacturing\r\nmethod with potential applications in engineering, medicine, science,\r\nand art. Printing ice at the micrometer–millimeter scale is\r\na challenging, high-frequency additive manufacturing process. Freeform\r\nice 3D printing though is an early stage manufacturing process where\r\ncomplex geometries require significant time to design manually through\r\nan empirical trial-and-error approach, due to the nonlinear nature\r\nof droplet deposition and solidification along with unavoidable uncertainties\r\nand noise. This process would be tremendously improved with better\r\nunderstanding and harnessing the ability to visualize, analyze and\r\nadjust the printing process in real time. For this automation, using\r\napproaches such as closed loop control that have allowed tremendous\r\nadvances in other fields like self-driving cars, plane autopilot,\r\netc. would be very useful. In order to use closed loop control for\r\nthis process, ice position and shape must be determined and transformed\r\ninto in situ actionable data, which is complicated further by the\r\ntransparency of ice and speed required for the phase transition from\r\nwater to ice. We implemented vision techniques to build a data set\r\nto train a machine learning algorithm though an ice segmentation approach\r\nusing a convolutional filter. We also implemented a hybrid optical\r\nflow algorithm (Farneback-FAST) to create a segmented video frame\r\ndata set for training a neural network, Icenet. This approach was\r\nfaster than the Farneback-FAST, segmenting frames in 25 ms, which\r\nallows for single and low multi droplet control. Our approaches will\r\nenable future closed loop control of the printing process and will\r\nbe useful in a variety of areas including additive manufacturing,\r\norgan on a chip systems, and biomanufacturing.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 6","pages":"1068–1075"},"PeriodicalIF":6.7000,"publicationDate":"2026-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical & Biomedical Imaging","FirstCategoryId":"1085","ListUrlMain":"https://pubs.acs.org/doi/10.1021/cbmi.5c00064","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2026/1/14 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Freeform 3D ice printing is emerging as a promising additive
manufacturing
method with potential applications in engineering, medicine, science,
and art. Printing ice at the micrometer–millimeter scale is
a challenging, high-frequency additive manufacturing process. Freeform
ice 3D printing though is an early stage manufacturing process where
complex geometries require significant time to design manually through
an empirical trial-and-error approach, due to the nonlinear nature
of droplet deposition and solidification along with unavoidable uncertainties
and noise. This process would be tremendously improved with better
understanding and harnessing the ability to visualize, analyze and
adjust the printing process in real time. For this automation, using
approaches such as closed loop control that have allowed tremendous
advances in other fields like self-driving cars, plane autopilot,
etc. would be very useful. In order to use closed loop control for
this process, ice position and shape must be determined and transformed
into in situ actionable data, which is complicated further by the
transparency of ice and speed required for the phase transition from
water to ice. We implemented vision techniques to build a data set
to train a machine learning algorithm though an ice segmentation approach
using a convolutional filter. We also implemented a hybrid optical
flow algorithm (Farneback-FAST) to create a segmented video frame
data set for training a neural network, Icenet. This approach was
faster than the Farneback-FAST, segmenting frames in 25 ms, which
allows for single and low multi droplet control. Our approaches will
enable future closed loop control of the printing process and will
be useful in a variety of areas including additive manufacturing,
organ on a chip systems, and biomanufacturing.
期刊介绍:
Chemical & Biomedical Imaging is a peer-reviewed open access journal devoted to the publication of cutting-edge research papers on all aspects of chemical and biomedical imaging. This interdisciplinary field sits at the intersection of chemistry physics biology materials engineering and medicine. The journal aims to bring together researchers from across these disciplines to address cutting-edge challenges of fundamental research and applications.Topics of particular interest include but are not limited to:Imaging of processes and reactionsImaging of nanoscale microscale and mesoscale materialsImaging of biological interactions and interfacesSingle-molecule and cellular imagingWhole-organ and whole-body imagingMolecular imaging probes and contrast agentsBioluminescence chemiluminescence and electrochemiluminescence imagingNanophotonics and imagingChemical tools for new imaging modalitiesChemical and imaging techniques in diagnosis and therapyImaging-guided drug deliveryAI and machine learning assisted imaging