MdRZFP34a promotes plant growth and enhances biotic stress resistance in apple.

IF 3.6 3区生物学 Q1 PLANT SCIENCES

Physiology and Molecular Biology of Plants Pub Date : 2026-06-01 Epub Date: 2026-05-19 DOI:10.1007/s12298-026-01763-x

Yi Zhao, Wenjun Chen, Chao Zhang, Yipeng Liu, Ziwen Wei, Zhihong Zhang, Hongyan Dai

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引用次数: 0

Abstract

Biotic stress poses a significant threat to apple growth and development, often leading to substantial economic losses in fruit production. RING-type E3 ubiquitin ligases play crucial roles in regulating various plant physiological processes, including hormone signaling, photomorphogenesis, carbohydrate metabolism, and responses to biotic stress. Notably, heterologous overexpression of CpLRR-RLK1 in Arabidopsis thaliana has been shown to enhance resistance to bacterial diseases. Transcriptomic analysis further revealed that the RING Zinc-Finger Protein 34 s (MdRZFP34a and MdRZFP34b) expression was significantly upregulated in the CpLRR-RLK1 transgenic apple lines. In this study, MdRZFP34a and MdRZFP34b were identified to contain conserved CHY zinc-finger and RING-related domains. Overexpression of MdRZFP34a significantly promoted lateral root development and vegetative growth in apple. Functional analysis demonstrated that MdRZFP34a positively regulates resistance to Colletotrichum fructicola infection and aphid colonization by enhancing the accumulation of antioxidant enzymes and the biosynthesis of secondary metabolites. These findings provide valuable insights into the biological mechanisms underlying MdRZFP34a-mediated biotic stress resistance in apple.

Supplementary information: The online version contains supplementary material available at 10.1007/s12298-026-01763-x.

查看原文本刊更多论文

MdRZFP34a促进苹果植株生长，增强生物抗逆性。

生物胁迫对苹果的生长发育构成重大威胁，往往导致果实生产的重大经济损失。环型E3泛素连接酶在调节多种植物生理过程中发挥重要作用，包括激素信号、光形态发生、碳水化合物代谢和对生物胁迫的反应。值得注意的是，在拟南芥中，外源过表达CpLRR-RLK1已被证明可以增强对细菌性疾病的抗性。转录组学分析进一步发现，环锌指蛋白34 s （MdRZFP34a和MdRZFP34b）在转CpLRR-RLK1的苹果株系中表达显著上调。本研究鉴定出MdRZFP34a和MdRZFP34b含有保守的CHY锌指结构域和ring相关结构域。过表达MdRZFP34a显著促进苹果侧根发育和营养生长。功能分析表明，MdRZFP34a通过增强抗氧化酶的积累和次生代谢产物的生物合成，积极调节对果炭疽菌感染和蚜虫定殖的抗性。这些发现为mdrzfp34a介导的苹果生物抗逆性的生物学机制提供了有价值的见解。补充信息：在线版本包含补充资料，下载地址为10.1007/s12298-026-01763-x。

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来源期刊

Physiology and Molecular Biology of Plants PLANT SCIENCES-

CiteScore

7.10

自引率

0.00%

发文量

126

期刊介绍： Founded in 1995, Physiology and Molecular Biology of Plants (PMBP) is a peer reviewed monthly journal co-published by Springer Nature. It contains research and review articles, short communications, commentaries, book reviews etc., in all areas of functional plant biology including, but not limited to plant physiology, biochemistry, molecular genetics, molecular pathology, biophysics, cell and molecular biology, genetics, genomics and bioinformatics. Its integrated and interdisciplinary approach reflects the global growth trajectories in functional plant biology, attracting authors/editors/reviewers from over 98 countries.