Ternary co-encapsulation in electrospun PVA fibers: release kinetics and mechanisms.

IF 3.4 Q2 PHARMACOLOGY & PHARMACY

Therapeutic delivery Pub Date : 2026-06-01 Epub Date: 2026-06-24 DOI:10.1080/20415990.2026.2692882

Andrea Ximena Esparza Monroy, Rebeca Betancourt Galindo, Antonio Serguei Ledezma Pérez, Arxel De León Santillán, Roberto Espinoza Neira, Carmen Natividad Alvarado Canché, Christian Chapa González, Perla Elvia García-Casillas

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引用次数: 0

Abstract

Aims: This study aimed to develop and evaluate a ternary co-encapsulation system based on electrospun poly(vinyl alcohol) (PVA) nanofibers containing Aloe vera extract, methylglyoxal (MGO), and collagen for multifunctional wound-healing applications with controlled therapeutic delivery.

Materials and methods: Electrospun nanofibers were characterized by scanning electron microscopy and FTIR spectroscopy. Swelling, degradation, and in vitro release profiles were evaluated under physiological conditions. Release kinetics were analyzed using Korsmeyer-Peppas and Weibull models. Antimicrobial activity against Escherichia coli and Staphylococcus aureus was assessed according to JIS Z 2801. Cytocompatibility was evaluated using human fibroblast cultures following ISO 10993-5 guidelines.

Results: The co-encapsulated nanofibers exhibited homogeneous morphology with fiber diameters of 350-500 nm and preserved bioactive agents´ integrity. The system showed a biphasic release profile, with rapid Aloe vera release (~80% within 4 h) followed by sustained MGO and collagen release up to 160 h. Release behavior involved coupled diffusion, polymer relaxation, and partial matrix dissolution. The ternary system demonstrated strong antimicrobial activity, achieving approximately 99.4% inhibition of E. coli, while maintaining excellent cytocompatibility with cell viability above 100%.

Conclusions: The ternary co-encapsulation strategy synergistic antimicrobial and regenerative effects through controlled multi-agent delivery in electrospun PVA wound-dressing platforms.

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静电纺PVA纤维的三元共包封：释放动力学和机制。

目的：本研究旨在开发和评估一种基于静电纺聚乙烯醇（PVA）纳米纤维的三元共包体系，该体系含有芦荟提取物、甲基乙二醛（MGO）和胶原蛋白，可用于控制治疗递送的多功能伤口愈合应用。材料与方法：采用扫描电镜和红外光谱对静电纺纳米纤维进行了表征。在生理条件下评估溶胀、降解和体外释放概况。采用Korsmeyer-Peppas和Weibull模型分析释放动力学。对大肠杆菌和金黄色葡萄球菌的抑菌活性按JIS z2801进行测定。按照ISO 10993-5指南使用人成纤维细胞培养物评估细胞相容性。结果：共包封的纳米纤维形态均匀，纤维直径在350 ~ 500 nm之间，并保持了生物活性物质的完整性。该系统显示出双相释放特征，芦荟快速释放（4小时内约80%），然后持续释放MGO和胶原蛋白长达160小时。释放行为包括耦合扩散、聚合物弛豫和部分基质溶解。该三元体系表现出较强的抗菌活性，对大肠杆菌的抑制率约为99.4%，同时保持良好的细胞相容性，细胞存活率在100%以上。结论：三元共包封策略通过控制多药递送在静电纺PVA创面敷料平台中协同抗菌和再生效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Therapeutic delivery PHARMACOLOGY & PHARMACY-

CiteScore

5.50

自引率

0.00%

发文量

期刊介绍： Delivering therapeutics in a way that is right for the patient - safe, painless, reliable, targeted, efficient and cost effective - is the fundamental aim of scientists working in this area. Correspondingly, this evolving field has already yielded a diversity of delivery methods, including injectors, controlled release formulations, drug eluting implants and transdermal patches. Rapid technological advances and the desire to improve the efficacy and safety profile of existing medications by specific targeting to the site of action, combined with the drive to improve patient compliance, continue to fuel rapid research progress. Furthermore, the emergence of cell-based therapeutics and biopharmaceuticals such as proteins, peptides and nucleotides presents scientists with new and exciting challenges for the application of therapeutic delivery science and technology. Successful delivery strategies increasingly rely upon collaboration across a diversity of fields, including biology, chemistry, pharmacology, nanotechnology, physiology, materials science and engineering. Therapeutic Delivery recognizes the importance of this diverse research platform and encourages the publication of articles that reflect the highly interdisciplinary nature of the field. In a highly competitive industry, Therapeutic Delivery provides the busy researcher with a forum for the rapid publication of original research and critical reviews of all the latest relevant and significant developments, and focuses on how the technological, pharmacological, clinical and physiological aspects come together to successfully deliver modern therapeutics to patients. The journal delivers this essential information in concise, at-a-glance article formats that are readily accessible to the full spectrum of therapeutic delivery researchers.