Detection and Characterization of ESBL-Producing and Carbapenem-Resistant Klebsiella pneumoniae in Ornamental Birds and Their Surrounding Environments.

IF 3.6 3区生物学 Q2 MICROBIOLOGY

MicrobiologyOpen Pub Date : 2026-06-01 DOI:10.1002/mbo3.70348

Md Abdullah Evna Hasan, Md Liton Rana, Md Saiful Islam, Sadia Afrin Punom, Md Tabeer Hossain Antor, Diruba Akter Jany, Naeem Ahammed Ibrahim Fahim, S M Abu Sama Al Faruquee, Md Raisul Islam, Zuhayr Bakhtiyar, Anindita Ash Prome, Md Monirul Islam, Saifur Rahman, Mohammad Ferdousur Rahman Khan, Md Tanvir Rahman

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引用次数: 0

Abstract

Extended-spectrum beta-lactamase-producing Klebsiella pneumoniae (ESBL-KP) and carbapenem-resistant Klebsiella pneumoniae (CRKP) represent a global health risk, with ornamental birds serving as potential reservoirs for their dissemination. This study aimed to isolate and characterize ESBL-KP and CRKP from ornamental birds and their surrounding environments. A total of 112 samples, including equal numbers of feces, cage swab, cage water, cage feed, store feed, handler's hand swab, nasal swab, and air samples, were collected equally from both households and pet bird shops. Overall, 52.68% (59/112, CI₉₅: 0.435-0.6168) isolates were confirmed as positive, where a higher occurrence was observed in pet store samples (32/56, 57.14%) than in household samples (27/56, 48.21%). In the disk diffusion test, 79.67% (47/59, CI₉₅: 0.6773-0.8796) isolates were identified as multidrug-resistant, with high resistance to imipenem and cefotaxime (both 66.10%), meropenem and ceftriaxone (each 59.02%). The occurrence of ESBL-producing isolates was higher in shop samples (26/32, 81.25%) than in household samples (12/27, 44.44%). In the Congo Red assay, 76.28% (45/59, CI₉₅: 0.6403-0.8531) isolates were identified as strong biofilm producers. Resistance genes, bla_CTX-M (92.10%), bla_TEM (84.21%), bla_SHV (78.95%), bla_OXA-51 (51.42%), and bla_NDM (34.29%), conferring resistance to beta-lactams and carbapenems were detected in the K. pneumoniae isolates, along with virulence genes, uge (100%), entB (83.05%), mrkD (81.36%), and kfu (30.51%). As a first report from Bangladesh, this study highlights the widespread occurrence of ESBL-KP, CRKP and biofilm-producing K. pneumoniae in ornamental birds, emphasizing the urgent need for a One Health approach to prevent associated zoonotic and environmental risks.

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观赏鸟类及其周围环境中产esbl和耐碳青霉烯肺炎克雷伯菌的检测与鉴定

产广谱β -内酰胺酶肺炎克雷伯菌（ESBL-KP）和耐碳青霉烯肺炎克雷伯菌（CRKP）是一种全球性的健康风险，观赏鸟类是其传播的潜在宿主。本研究旨在从观赏鸟类及其周围环境中分离和鉴定ESBL-KP和CRKP。从家庭和宠物鸟店平均收集粪便、笼拭子、笼水、笼饲料、商店饲料、操作人员手拭子、鼻拭子和空气样本共112份。总体上，52.68% （59/112,CI95: 0.435 ~ 0.6168）的分离株被证实为阳性，其中宠物店样本（32/56,57.14%）高于家庭样本（27/56,48.21%）。盘片扩散试验中，多重耐药菌株占79.67% (47/59,CI95: 0.6773 ~ 0.8796)，其中对亚胺培南和头孢噻肟的耐药率均为66.10%，对美罗培南和头孢曲松的耐药率均为59.02%。店铺样本中产生esbl的分离株的发生率（26/32,81.25%）高于家庭样本（12/27,44.44%）。在刚果红试验中，76.28% （45/59,CI95: 0.6403-0.8531）分离株被鉴定为强生物膜产生菌。检出耐药基因blaCTX-M（92.10%）、blaTEM（84.21%）、blaSHV（78.95%）、blaOXA-51（51.42%）和blaNDM(34.29%)，以及毒力基因uge（100%）、entB（83.05%）、mrkD（81.36%）和kfu（30.51%）。作为孟加拉国的第一份报告，这项研究强调了ESBL-KP、CRKP和产生生物膜的肺炎克雷伯菌在观赏鸟类中的广泛存在，强调了迫切需要采取“同一个健康”方法来预防相关的人畜共患病和环境风险。

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来源期刊

MicrobiologyOpen MICROBIOLOGY-

CiteScore

8.00

自引率

0.00%

发文量

审稿时长

20 weeks

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