{"title":"Effect of an oxygen-releasing mouthwash on <i>Aggregatibacter actinomycetemcomitans</i> and HaCaT cells: A pilot study.","authors":"Ayyanahalli Matta Bharath Kumar, Swastik Mishra, Pratibha Gopalkrishna, Santhosh Kumar","doi":"10.4103/jisp.jisp_418_24","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Oxygen-releasing oral care products are effective in treating ulcers, gingival disease, and root canal irrigation. The present <i>in vitro</i> study evaluated the antibacterial properties and biological effects of a commercially available oxygen-releasing mouthwash, Blue<sup>®</sup>M.</p><p><strong>Materials and methods: </strong>We compared Blue<sup>®</sup>M mouthwash with chlorhexidine (CHX) using the agar disk diffusion assay and minimum inhibitory concentration methods. Furthermore, 1 μL/mL of mouthwash was applied to HaCaT cells to assess its effects on cell migration using the scratch migration assay.</p><p><strong>Results: </strong>The zone of inhibition for the two mouthwash formulations was comparable. Blue<sup>®</sup>M mouthwash demonstrated the inhibition of <i>Aggregatibacter actinomycetemcomitans</i> (Aa) at 6.25 μg/mL, and 0.12% CHX was effective at 0.4 μg/mL. Blue<sup>®</sup>M (1 μL/mL) promoted HaCaT cell migration by up to 88.5% at 72 h compared to the control, with no adverse morphological changes.</p><p><strong>Conclusions: </strong>Blue<sup>®</sup>M was effective at inhibiting Aa and exhibited negligible cytotoxicity in HaCaT cells, with minimal effects on cell migration.</p>","PeriodicalId":15890,"journal":{"name":"Journal of Indian Society of Periodontology","volume":"30 1","pages":"73-79"},"PeriodicalIF":0.0000,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13286538/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Indian Society of Periodontology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/jisp.jisp_418_24","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2026/5/13 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"Dentistry","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Oxygen-releasing oral care products are effective in treating ulcers, gingival disease, and root canal irrigation. The present in vitro study evaluated the antibacterial properties and biological effects of a commercially available oxygen-releasing mouthwash, Blue®M.
Materials and methods: We compared Blue®M mouthwash with chlorhexidine (CHX) using the agar disk diffusion assay and minimum inhibitory concentration methods. Furthermore, 1 μL/mL of mouthwash was applied to HaCaT cells to assess its effects on cell migration using the scratch migration assay.
Results: The zone of inhibition for the two mouthwash formulations was comparable. Blue®M mouthwash demonstrated the inhibition of Aggregatibacter actinomycetemcomitans (Aa) at 6.25 μg/mL, and 0.12% CHX was effective at 0.4 μg/mL. Blue®M (1 μL/mL) promoted HaCaT cell migration by up to 88.5% at 72 h compared to the control, with no adverse morphological changes.
Conclusions: Blue®M was effective at inhibiting Aa and exhibited negligible cytotoxicity in HaCaT cells, with minimal effects on cell migration.
期刊介绍：
The Journal of Indian Society of Periodontology publishes original scientific articles to support practice , education and research in the dental specialty of periodontology and oral implantology. Journal of Indian Society of Periodontology (JISP), is the official publication of the Society and is managed and brought out by the Editor of the society. The journal is published Bimonthly with special issues being brought out for specific occasions. The ISP had a bulletin as its publication for a large number of years and was enhanced as a Journal a few years ago
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