In vitro analysis of a glass solder matrix on ATZ-ceramic-samples using human dental pulp cells and L929 mouse fibroblasts.

IF 4.7 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

International Journal of Implant Dentistry Pub Date : 2026-06-24 DOI:10.1186/s40729-026-00697-z

Sandra Fuest, Moritz L Dyck, Mustafa Becerikli, Andreas Schmoock, Anders Henningsen, Christian Moss, Robert Mau, Hermann Seitz, Reinhard E Friedrich, Martin Gosau, Ralf Smeets, Levi Matthies

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引用次数: 0

Abstract

Purpose: Optimized surface properties are a pivotal factor for dental ceramic implants and overall implant success. After grit-blasting and spray-coating Aluminum Toughened Zirconia (ATZ) ceramic samples with a glass solder, the biological cell response was examined and compared to uncoated and solely grit-blasted samples of the same ceramic.

Methods: In this study, a silica-based glass solder was evaluated for cytocompatibility, osteogenic differentiation, and hemocompatibility using L929 mouse fibroblasts and human dental pulp cells (HDPCs).

Results: No toxic effects were observed for either coated or uncoated ATZ samples in direct or indirect tests, as assessed by live/dead staining. Differentiation and viability assays with HDPCs and L929 mouse fibroblasts showed no toxicity, and osteogenic differentiation was not impaired by the coating. Hemocompatibility testing with human whole blood revealed similar results for coated and uncoated ceramic specimens, with no hemolysis or adverse effects on standard hematological parameters.

Conclusions: The glass solder coating was cytocompatible and hemocompatible under the tested in vitro conditions and did not impede osteogenic differentiation. These findings indicate that it can serve as a suitable substrate for the proliferation and spreading of L929 fibroblasts and HDPCs, with potential benefits for improving dental ceramic implant surfaces.

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利用人牙髓细胞和L929小鼠成纤维细胞体外分析atz -陶瓷样品上的玻璃焊料基质。

目的：牙体表面性能的优化是牙体种植成功的关键因素。在喷砂和喷涂带有玻璃焊料的铝增韧氧化锆（ATZ）陶瓷样品后，研究了生物细胞的响应，并将其与未涂砂和单独喷砂的相同陶瓷样品进行了比较。方法：利用L929小鼠成纤维细胞和人牙髓细胞（HDPCs）对硅基玻璃焊料的细胞相容性、成骨分化和血液相容性进行了评价。结果：在直接或间接测试中，通过活/死染色评估，没有观察到包被或未包被的ATZ样品的毒性作用。HDPCs和L929小鼠成纤维细胞的分化和活力实验显示没有毒性，并且成骨分化不受涂层的影响。人体全血的血液相容性测试显示，涂层和未涂层陶瓷样品的结果相似，没有溶血或对标准血液学参数的不良影响。结论：在体外实验条件下，玻璃焊料涂层具有细胞相容性和血液相容性，不影响成骨分化。这些结果表明，它可以作为L929成纤维细胞和HDPCs增殖和扩散的合适底物，具有改善牙科陶瓷种植体表面的潜在益处。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Implant Dentistry DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

1.70

自引率

7.40%

发文量

审稿时长

13 weeks

期刊介绍： The International Journal of Implant Dentistry is a peer-reviewed open access journal published under the SpringerOpen brand. The journal is dedicated to promoting the exchange and discussion of all research areas relevant to implant dentistry in the form of systematic literature or invited reviews, prospective and retrospective clinical studies, clinical case reports, basic laboratory and animal research, and articles on material research and engineering.