{"title":"Association of IBS-associated E. coli strains with epithelial inflammatory responses and tight junction dysregulation in Caco-2 cells.","authors":"Zeinab Merzaei, Hossein Kazemian, Zahra Bamzadeh, Mansoureh Paknejadi, Ebrahim Kouhsari","doi":"10.1186/s13104-026-07924-1","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Irritable bowel syndrome (IBS) presents with symptoms of pain, abdominal discomfort, and altered bowel habits. Changes in the intestinal microbiota, including an increase in Enterobacteriaceae like E. coli, have been associated with IBS. The aim of this study was to evaluate the effects of IBS-associated E. coli strains on epithelial inflammatory responses, tight junction-related gene expression, and Toll-like receptor regulation in Caco-2 cells.</p><p><strong>Results: </strong>Results showed that IBS-associated E. coli strains exhibited significantly higher invasion activity and increased production of IL-8 and TNF-α compared to H-E. coli. Furthermore, IBS-associated E. coli strains upregulated TLR-4 and TLR-5 expression and reduced the expression of tight junction proteins.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7000,"publicationDate":"2026-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13386935/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC Research Notes","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/s13104-026-07924-1","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: Irritable bowel syndrome (IBS) presents with symptoms of pain, abdominal discomfort, and altered bowel habits. Changes in the intestinal microbiota, including an increase in Enterobacteriaceae like E. coli, have been associated with IBS. The aim of this study was to evaluate the effects of IBS-associated E. coli strains on epithelial inflammatory responses, tight junction-related gene expression, and Toll-like receptor regulation in Caco-2 cells.
Results: Results showed that IBS-associated E. coli strains exhibited significantly higher invasion activity and increased production of IL-8 and TNF-α compared to H-E. coli. Furthermore, IBS-associated E. coli strains upregulated TLR-4 and TLR-5 expression and reduced the expression of tight junction proteins.
BMC Research NotesBiochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
3.60
自引率
0.00%
发文量
363
审稿时长
15 weeks
期刊介绍：
BMC Research Notes publishes scientifically valid research outputs that cannot be considered as full research or methodology articles. We support the research community across all scientific and clinical disciplines by providing an open access forum for sharing data and useful information; this includes, but is not limited to, updates to previous work, additions to established methods, short publications, null results, research proposals and data management plans.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
