The developmental pattern of calcium mobilisation during embryogenesis in Muscovy ducks.

IF 1.7 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

British Poultry Science Pub Date : 2026-06-24 DOI:10.1080/00071668.2026.2667999

X Wang, Y Ren, Q Hu, Q Wu, G Sun, H Chen, J Lin, S Liu, W Wang, J Qin, Y Zhu

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引用次数: 0

Abstract

1. Calcium (Ca) is essential for avian embryonic bone mineralisation, yet its metabolic dynamics during duck embryogenesis remain unclear. This study investigated Ca mobilisation in Muscovy duck embryos.2. Eggshell (ES) and yolk samples were collected from embryonic day (ED) 16 to ED31 for analysis of ES physical properties (thickness, strength, mass ratio) and Ca levels. Serum, yolk sac membrane (YSM) and liver tissues were collected from ED16 to the day of hatch (DOH) to assess serum biochemical parameters and relative mRNA expression of genes associated with Ca transport (PLCG1, PRKCA, CALM1) and absorption (CALB1, ARNT, EP300).3. Results showed ES quality and Ca levels decreased (p < 0.01) linearly with increasing incubation days. Yolk Ca levels decreased (p < 0.01) linearly and quadratically, reaching a minimum on ED25. Serum Ca levels and alkaline phosphatase (ALP) activity increased (p < 0.01) linearly and quadratically during ED16-DOH and achieved a plateau during ED28-DOH. Expression of Ca-related genes in YSM and liver increased quadratically (p < 0.01), peaking during ED25-28.4. In conclusion, ES quality declined with reduced Ca levels during embryogenesis. Concurrently, yolk Ca levels, along with serum Ca and ALP levels, increased significantly during ED25-28, indicating peak embryonic Ca mobilisation during this period. Additionally, relative mRNA expression of Ca mobilisation-related genes in the YSM and liver exhibited a similar increasing trend. This suggested enhanced Ca mobilisation during this phase to support bone mineralisation and growth in duck embryos.

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番鸭胚胎期钙动员的发育模式。

1. 钙（Ca）是鸟类胚胎骨矿化所必需的，但其在鸭胚胎发生过程中的代谢动力学尚不清楚。本研究对番鸭胚胎钙的动员进行了研究。取胚胎第16 ~ 31天蛋壳和蛋黄样品，分析蛋壳和蛋黄的物理性质（厚度、强度、质量比）和钙水平。从ED16到孵化日（DOH）收集血清、卵黄囊膜（YSM）和肝脏组织，评估血清生化参数和钙转运相关基因（PLCG1、PRKCA、CALM1）和钙吸收相关基因（CALB1、ARNT、EP300）的相对mRNA表达量。结果显示ES质量和Ca水平下降（p p p p）

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

British Poultry Science 农林科学-奶制品与动物科学

CiteScore

3.90

自引率

5.00%

发文量

审稿时长

4.5 months

期刊介绍： From its first volume in 1960, British Poultry Science has been a leading international journal for poultry scientists and advisers to the poultry industry throughout the world. Over 60% of the independently refereed papers published originate outside the UK. Most typically they report the results of biological studies with an experimental approach which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: anatomy, embryology, biochemistry, biophysics, physiology, reproduction and genetics, behaviour, microbiology, endocrinology, nutrition, environmental science, food science, feeding stuffs and feeding, management and housing welfare, breeding, hatching, poultry meat and egg yields and quality.Papers that adopt a modelling approach or describe the scientific background to new equipment or apparatus directly relevant to the industry are also published. The journal also features rapid publication of Short Communications. Summaries of papers presented at the Spring Meeting of the UK Branch of the WPSA are published in British Poultry Abstracts .