{"title":"Genomic characterization of three novel Shiga toxin-producing Escherichia coli lytic phages","authors":"AE Juarez, A Krüger, PMA Lucchesi","doi":"10.1007/s00705-026-06673-7","DOIUrl":null,"url":null,"abstract":"<div><p>Shiga toxin-producing <i>Escherichia coli</i> (STEC) are major foodborne pathogens. In response to the need for safe biocontrol strategies, lytic phages have emerged as promising tools for STEC control. This study analyses genomic features of three novel phages, L73, PG3 and A4.3, isolated from cattle farms using STEC strains as hosts. L73 and PG3 were classified as <i>Tequatrovirus</i>, while A4.3 as <i>Vequintavirus</i>. Genomic analyses revealed no markers of lysogeny, antimicrobial resistance, or virulence. Phylogenetic and comparative analyses confirmed close relationships to reference phages of each genus, while also revealing distinct genetic differences. These phages constitute genomically well-supported candidates for future evaluation as STEC biocontrol agents.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 7","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2026-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of Virology","FirstCategoryId":"3","ListUrlMain":"https://link.springer.com/article/10.1007/s00705-026-06673-7","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VIROLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Shiga toxin-producing Escherichia coli (STEC) are major foodborne pathogens. In response to the need for safe biocontrol strategies, lytic phages have emerged as promising tools for STEC control. This study analyses genomic features of three novel phages, L73, PG3 and A4.3, isolated from cattle farms using STEC strains as hosts. L73 and PG3 were classified as Tequatrovirus, while A4.3 as Vequintavirus. Genomic analyses revealed no markers of lysogeny, antimicrobial resistance, or virulence. Phylogenetic and comparative analyses confirmed close relationships to reference phages of each genus, while also revealing distinct genetic differences. These phages constitute genomically well-supported candidates for future evaluation as STEC biocontrol agents.
期刊介绍：
Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.
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