Functional Diversity and Community Composition of Soil Fungi Associated With Canopy Dieback in Araucaria araucana Forests of Contrasting Edaphic Conditions.

IF 3.1 4区生物学 Q2 ENVIRONMENTAL SCIENCES

Environmental Microbiology Reports Pub Date : 2026-06-01 DOI:10.1111/1758-2229.70361

Valeria Arriagada, Adrian Garrido, Francisca Madariaga, Rodrigo Hasbun, Eugenio Sanfuentes

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引用次数: 0

Abstract

Background: Soil fungal communities play vital roles in forest ecosystem functioning, yet their relationship with tree health remains insufficiently characterized in many endangered species.

Aims: This study, investigated the composition and functional structure of soil fungi associated with symptomatic and asymptomatic individuals of Araucaria araucana in the Nahuelbuta Coastal Range, Chile.

Materials and methods: Using high-throughput ITS1 sequencing and trait-based annotation, we compared fungal assemblages across two forest sectors with contrasting edaphic conditions.

Results: Marked differences in taxonomic and functional composition were observed between sites, with more even and functionally diverse communities in less restrictive soils. Within this site, where both tree health conditions co-occur, no significant differences in alpha or beta diversity were detected; nevertheless, species-level and functional guild analyses revealed shifts in composition. A shared core microbiome included taxa putatively identified as endophytes, including Cladophialophora minutissima, Fraxinicola europaea, Linnemannia hyalina, saprotrophs (Solicoccozyma terricola, Helicodendron conglomeratum, Pseudogymnoascus roseus) and a plant pathogen (Penicillium excelsum). Symptomatic trees harboured unique stress-tolerant taxa, including cold-adapted saprotrophs and ericoid mycorrhizal fungi, while asymptomatic trees supported lignocellulose decomposers, mutualists and early-successional symbionts.

Discussion: The observed fungal shifts suggest that canopy dieback is associated with a functional reorganization of the rhizosphere microbiome rather than a simple loss of diversity.

Conclusion: These findings provide new insights into the fungal ecology of A. araucana forest and contribute to understanding how soil microbiomes respond to forest decline under contrasting edaphic conditions.

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不同土壤条件下杉木林冠层枯死相关土壤真菌的功能多样性和群落组成

背景：土壤真菌群落在森林生态系统功能中起着至关重要的作用，但它们与树木健康的关系在许多濒危物种中仍未得到充分的研究。目的：研究智利Nahuelbuta海岸山脉Araucaria araucana有症状和无症状个体的土壤真菌组成和功能结构。材料和方法：利用高通量ITS1测序和基于性状的注释，我们比较了不同土壤条件下两个森林部门的真菌组合。结果：不同地点间植物群落在分类和功能组成上存在显著差异，在限制性较弱的土壤中群落分布更为均匀和功能多样化。在这两种树木健康状况同时发生的地点，没有检测到α或β多样性的显著差异；然而，物种水平和功能协会分析揭示了组成的变化。共享的核心微生物群包括被认为是内生菌的分类群，包括Cladophialophora minutissima、Fraxinicola europaea、Linnemannia hyalina、腐养菌（陆地副球菌、团绒Helicodendron glomeratum、Pseudogymnoascus roseus）和植物病原菌（青霉菌）。有症状的树木拥有独特的抗逆性分类群，包括适应冷的腐养菌和ericoid菌根真菌，而无症状的树木则支持木质纤维素分解者、共生菌和早期演替共生体。讨论：观察到的真菌转移表明，冠层枯死与根际微生物群的功能重组有关，而不是简单的多样性丧失。结论：这些研究结果为深入了解杉木森林真菌生态学提供了新的思路，有助于了解不同土壤条件下土壤微生物组对森林退化的响应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Environmental Microbiology Reports ENVIRONMENTAL SCIENCES-MICROBIOLOGY

CiteScore

6.00

自引率

3.00%

发文量

审稿时长

3.0 months

期刊介绍： The journal is identical in scope to Environmental Microbiology, shares the same editorial team and submission site, and will apply the same high level acceptance criteria. The two journals will be mutually supportive and evolve side-by-side. Environmental Microbiology Reports provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following: the structure, activities and communal behaviour of microbial communities microbial community genetics and evolutionary processes microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors microbes in the tree of life, microbial diversification and evolution population biology and clonal structure microbial metabolic and structural diversity microbial physiology, growth and survival microbes and surfaces, adhesion and biofouling responses to environmental signals and stress factors modelling and theory development pollution microbiology extremophiles and life in extreme and unusual little-explored habitats element cycles and biogeochemical processes, primary and secondary production microbes in a changing world, microbially-influenced global changes evolution and diversity of archaeal and bacterial viruses new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens.