Smartphone-integrated bifunctional sensing platform for visual detection of ampicillin and tetracycline in animal manure.

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2026-12-01 Epub Date: 2026-06-21 DOI:10.1016/j.talanta.2026.130173

Ming-Ming Chen, Lu-Chen Cheng, Xiao-Yu Dong, Fang-Jie Zhao, Peng Wang

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引用次数: 0

Abstract

Antibiotic residues in environmental matrices pose escalating threats to ecosystems and human health, creating demand for rapid detection. Here, we present a portable smartphone-integrated bifunctional sensor based on Fe-CDs that enables sequential colorimetric and fluorescent detection of ampicillin (Amp) and tetracycline (TC). The Fe-CDs, synthesized via a one-pot hydrothermal process, exhibit stable fluorescence and intrinsic peroxidase-mimicking activity. Ampicillin inhibits the Fe-CDs nanozyme-catalyzed TMB/H₂O₂ chromogenic reaction via •OH consumption, allowing visual colorimetric quantification. Meanwhile, tetracycline strongly quenches the fluorescent signal of Fe-CDs through an inner filter effect, enabling a sensitive fluorescence readout. This bifunctional assay achieves high sensitivity (LOD as low as ∼0.012 μg/mL for Amp and 0.005 μg/mL for TC). By incorporating Fe-CDs onto paper test strips and analyzing with smartphone cameras, the platform allows real-time, rapid quantification of antibiotics in complex matrices. In manure samples, the sensor's results (recoveries ∼94.4-104.8%) agree well with LC-MS/MS measurements, underscoring its accuracy and robustness. Overall, the portable bifunctional Fe-CDs sensor provides a sensitive, field-deployable tool for environmental antibiotic surveillance.

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基于智能手机集成的动物粪便氨苄西林和四环素双功能视觉检测平台。

环境基质中的抗生素残留对生态系统和人类健康构成日益严重的威胁，因此需要快速检测。在这里，我们提出了一种基于Fe-CDs的便携式智能手机集成双功能传感器，可以进行氨苄西林（Amp）和四环素（TC）的顺序比色和荧光检测。通过一锅水热法合成的Fe-CDs具有稳定的荧光和固有的过氧化物酶模拟活性。氨苄西林通过•OH消耗抑制Fe-CDs纳米酶催化的TMB/H2O2显色反应，允许视觉比色定量。同时，四环素通过内部滤光效应强烈猝灭Fe-CDs的荧光信号，从而实现灵敏的荧光读出。该双功能检测具有高灵敏度（Amp的LOD低至~ 0.012 μg/mL， TC的LOD低至0.005 μg/mL）。通过将Fe-CDs整合到纸质试纸上，并使用智能手机摄像头进行分析，该平台可以实时、快速地定量复杂基质中的抗生素。在粪便样品中，传感器的结果（回收率~ 94.4-104.8%）与LC-MS/MS测量结果一致，强调了其准确性和稳健性。总体而言，便携式双功能Fe-CDs传感器为环境抗生素监测提供了一种敏感的、可现场部署的工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.