Colorimetric swab and turbidimetric sensing for dual-mode point-of-use testing of demeton pesticide in foods based on oxidase-like Cu₂O@Ag nanoprobe.

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2026-12-01 Epub Date: 2026-06-20 DOI:10.1016/j.talanta.2026.130192

Yi Liu, Yinxia Yang, Jialin Liang, Lei Han, Qian Zhao, Jie Luo, Shi Gang Liu

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引用次数: 0

Abstract

Dual-mode sensing can enhance detection reliability by cross-validating results from distinct signals. Herein, a colorimetric and turbidimetric dual-mode sensing platform based on Cu₂O@Ag nanozyme with robust oxidase-like activity is developed for point-of-use (POU) detection of demeton. The Cu₂O@Ag efficiently catalyzes the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB), producing concurrent absorbance and turbidity signals. Demeton blocks active sites and suppresses catalytic activity in a concentration-dependent manner. Based on the principle, two modes for POU testing of demeton are developed. The first mode is based on a smartphone-assisted colorimetric swab and shows a linear range of 50 - 4000 ng/mL with the detection limit of 0.190 ng/mL. The other is based on a portable turbidimeter and exhibits a linear detection range of 50 to 3000 ng/mL with a detection limit of 2.997 ng/mL. The approaches are successfully validated in real food samples including baby cabbage, lettuce, cilantro, and tea leaves. This work presents a rapid, low-cost, and instrument-flexible dual-mode sensing strategy for on-site analysis of demeton residue. Also, the dual-mode detection based on colorimetric and turbidimetric signals for the first time offers a novel insight for dual-mode sensing.

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基于氧化酶样Cu2O@Ag纳米探针的比色拭子和浊度传感在食品中双模式使用点检测中的应用

双模检测通过对不同信号的检测结果进行交叉验证，提高了检测的可靠性。本文开发了一种基于Cu2O@Ag纳米酶的比色和比浊双模式传感平台，该平台具有强大的氧化酶样活性，用于使用点（POU）检测demeton。Cu2O@Ag有效催化3,3',5,5'-四甲基联苯胺（TMB）的氧化，同时产生吸光度和浊度信号。Demeton阻断活性位点并以浓度依赖的方式抑制催化活性。在此基础上，建立了两种测试模式。第一种模式基于智能手机辅助比色棉签，线性范围为50 - 4000 ng/mL，检出限为0.190 ng/mL。另一种是基于便携式浊度计，线性检测范围为50 ~ 3000 ng/mL，检出限为2.997 ng/mL。这些方法已经在真实的食物样品中得到了成功的验证，包括小卷心菜、生菜、香菜和茶叶。本工作提出了一种快速、低成本、仪器灵活的双模传感策略，用于现场分析土墨顿残留物。此外，基于比色和浊度信号的双模检测首次为双模传感提供了新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.