Eco-Friendly Engineered Ternary Nanocomposite for Label-Free Fluorescent SARS-CoV-2 N-Gene DNA Detection

IF 7.5 Q2 NANOSCIENCE & NANOTECHNOLOGY

ACS Nanoscience Au Pub Date : 2026-06-17 Epub Date: 2026-02-19 DOI:10.1021/acsnanoscienceau.5c00179

Manikandan Dhayalan, Mallu Chenna Reddy, Madan Kumar Arumugam, Desy Liana, Billy Wai-Lung Ng, Anuchit Phanumartwiwath

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引用次数: 0

Abstract

The COVID-19 pandemic brought to light the demand for nucleic-acid sensing platforms that are rapid, resilient, and scalable for integration at the point-of-care. In this study, we described a green-engineered fluorescence biosensing platform using a Ag–TiO₂–CeO₂ ternary nanocomposite fabricated with Morinda citrifolia leaf extract. The biosynthetic approach facilitated the production of a nanocomposite that is biocompatible, exhibits high surface area, and contains oxygen vacancy related to surface sites that promote favorable interactions with nucleic acids. The nanocomposite was functionalized with thiol-terminated antisense probes targeting the SARS-CoV-2 N-gene and employed in enzyme-free hybridization assays, using synthetic DNA as a model target and human genomic DNA as a model for nonspecific hybridization. The platform showed a significant shift in fluorescence after 30 min of hybridization and was capable of detecting target unlabeled DNA at a concentration of 3 pM. The interaction of the nanocomposite with short DNA oligonucleotides and long double-stranded genomic DNA showed the potential of the surface chemistry of the nanocomposite for nucleic acid sensing. This study provides a nanocomposite fluorescence biosensing platform at the material proof level and paves the way for future extensions and viral diagnostics in complex biological mixtures.

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用于无标记SARS-CoV‑2 N‑基因DNA荧光检测的环保工程三元纳米复合材料

2019冠状病毒病大流行凸显了对快速、有弹性和可扩展的核酸检测平台的需求，以便在护理点进行整合。在这项研究中，我们描述了一种绿色工程荧光生物传感平台，该平台使用的是由桑叶提取物制成的Ag-TiO2-CeO2三元纳米复合材料。生物合成方法促进了纳米复合材料的生产，这些纳米复合材料具有生物相容性，具有高表面积，并且含有与促进与核酸有利相互作用的表面位点相关的氧空位。利用巯基端反义探针靶向SARS-CoV-2 n基因功能化纳米复合材料，并以合成DNA为模型靶点，以人类基因组DNA为非特异性杂交模型，进行无酶杂交试验。杂交30分钟后，该平台显示出明显的荧光变化，并且能够检测浓度为3pm的目标未标记DNA。纳米复合材料与短DNA寡核苷酸和长双链基因组DNA的相互作用显示了纳米复合材料在核酸传感方面的表面化学潜力。这项研究提供了一个材料证明水平的纳米复合荧光生物传感平台，为未来在复杂生物混合物中的扩展和病毒诊断铺平了道路。

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来源期刊

ACS Nanoscience Au 材料科学、纳米科学-

CiteScore

4.20

自引率

0.00%

发文量

期刊介绍： ACS Nanoscience Au is an open access journal that publishes original fundamental and applied research on nanoscience and nanotechnology research at the interfaces of chemistry biology medicine materials science physics and engineering.The journal publishes short letters comprehensive articles reviews and perspectives on all aspects of nanoscience and nanotechnology:synthesis assembly characterization theory modeling and simulation of nanostructures nanomaterials and nanoscale devicesdesign fabrication and applications of organic inorganic polymer hybrid and biological nanostructuresexperimental and theoretical studies of nanoscale chemical physical and biological phenomenamethods and tools for nanoscience and nanotechnologyself- and directed-assemblyzero- one- and two-dimensional materialsnanostructures and nano-engineered devices with advanced performancenanobiotechnologynanomedicine and nanotoxicologyACS Nanoscience Au also publishes original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials engineering physics bioscience and chemistry into important applications of nanomaterials.