TRIM31: A Novel Guardian Against Periodontal Inflammation via Modulation of NLRP3 Inflammasome and Macrophage Polarization.

IF 2.3 4区医学 Q3 IMMUNOLOGY

Immunological Investigations Pub Date : 2026-10-01 Epub Date: 2026-06-22 DOI:10.1080/08820139.2026.2680504

Xiaolu Zhang, Dazhuang Lu, Lijia Lin, Weijuan Qi, Shan He

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引用次数: 0

Abstract

Background: Periodontitis involves dysregulated immunity where the NLRP3 inflammasome plays a key role, while the role of TRIM31, an E3 ubiquitin ligase, remains unknown in periodontitis.

Methods: Human gingival fibroblasts (HGFs) and macrophages were stimulated with LPS and ATP; TRIM31 was overexpressed via AAV, and NLRP3 was knocked out via CRISPR; periodontitis was induced in WT and NLRP3-KO mice treated with AAV-TRIM31; bone loss, osteoclasts, and apoptosis were assessed.

Results: TRIM31 was downregulated in inflammation and correlated with M2 polarization. TRIM31 overexpression protected HGFs, promoted M2 polarization, and bound to NLRP3, thereby promoting K48-linked ubiquitination and degradation. In vivo, TRIM31 reduced bone loss, osteoclasts, and apoptosis; these effects were abolished in NLRP3-KO cells and mice.

Conclusion: TRIM31 negatively regulates periodontal inflammation via ubiquitin-dependent NLRP3 degradation.

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TRIM31：通过调节NLRP3炎性体和巨噬细胞极化来对抗牙周炎症的新守护者。

背景：牙周炎涉及免疫失调，其中NLRP3炎性体起关键作用，而TRIM31 （E3泛素连接酶）在牙周炎中的作用尚不清楚。方法：LPS和ATP刺激人牙龈成纤维细胞（HGFs）和巨噬细胞；TRIM31通过AAV过表达，NLRP3通过CRISPR敲除；AAV-TRIM31诱导WT和NLRP3-KO小鼠牙周炎；评估骨丢失、破骨细胞和细胞凋亡。结果：TRIM31在炎症中下调，与M2极化相关。TRIM31过表达可保护hgf，促进M2极化，并与NLRP3结合，从而促进k48相关的泛素化和降解。在体内，TRIM31可减少骨质流失、破骨细胞和细胞凋亡；这些作用在NLRP3-KO细胞和小鼠中被消除。结论：TRIM31通过降解泛素依赖性NLRP3负向调节牙周炎症。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Immunological Investigations 医学-免疫学

CiteScore

5.50

自引率

7.10%

发文量

审稿时长

3 months

期刊介绍： Disseminating immunological developments on a worldwide basis, Immunological Investigations encompasses all facets of fundamental and applied immunology, including immunohematology and the study of allergies. This journal provides information presented in the form of original research articles and book reviews, giving a truly in-depth examination of the latest advances in molecular and cellular immunology.