The role and predictive potential of miR-451-5p in delayed fracture healing.

IF 2.2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Cytotechnology Pub Date : 2026-08-01 Epub Date: 2026-06-19 DOI:10.1007/s10616-026-00994-w

Chuangye Qu, Weibing Bao, Wenqi Wang, Xiaozhong Zhu

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引用次数: 0

Abstract

To explore the predictive value of miR-451-5p in delayed fracture healing (DFH) and its mechanism of action in fracture healing. Patients with normal fracture healing (NFH) and DFH were included in this study. The expression of miR-451-5p in serum was detected by RT-qPCR. The predictive potential of miR-451-5p for DFH was evaluated by ROC analysis, and risk factors influencing DFH were determined using logistic regression analysis. The proliferation, apoptosis and expression levels of osteogenic differentiation markers (ALP, BMP2, OSX) of mouse embryonic osteoblast precursor cells (MC3T3-E1) were detected by CCK-8, flow cytometry, RT-qPCR and western blot. The targeting relationship between miR-451-5p and ATF2 was verified by dual-luciferase reporter assay and RNA pull-down experiments. The level of miR-451-5p in the serum of DFH patients was significantly higher than that in the NFH group. Its sensitivity for predicting DFH was 81.33%, and its specificity was 86.96%. miR-451-5p is an independent risk factor for DFH. Overexpression of miR-451-5p could inhibit the proliferation of osteogenic precursor cells, promote cell apoptosis, and downregulate the expression of osteogenic differentiation markers. miR-451-5p binds to ATF2 and inhibits its expression. Overexpression of ATF2 could reverse the inhibitory effect of miR-451-5p on osteogenic differentiation. During the process of osteogenesis, miR-451-5p negatively regulates cell proliferation and osteogenesis by inhibiting the function of ATF2. Clinically, miR-451-5p has the potential to serve as a biomarker for the early predictive of DFH.

Supplementary information: The online version contains supplementary material available at 10.1007/s10616-026-00994-w.

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miR-451-5p在延迟骨折愈合中的作用和预测潜力。

探讨miR-451-5p对骨折延迟愈合（DFH）的预测价值及其在骨折愈合中的作用机制。本研究包括正常骨折愈合（NFH）和DFH患者。RT-qPCR检测血清中miR-451-5p的表达。采用ROC分析评估miR-451-5p对DFH的预测潜力，采用logistic回归分析确定影响DFH的危险因素。采用CCK-8、流式细胞术、RT-qPCR和western blot检测小鼠胚胎成骨前体细胞（MC3T3-E1）的增殖、凋亡及成骨分化标志物（ALP、BMP2、OSX）的表达水平。通过双荧光素酶报告基因实验和RNA下拉实验验证miR-451-5p与ATF2的靶向关系。DFH患者血清中miR-451-5p水平明显高于NFH组。预测DFH的敏感性为81.33%，特异性为86.96%。miR-451-5p是DFH的独立危险因素。过表达miR-451-5p可抑制成骨前体细胞的增殖，促进细胞凋亡，下调成骨分化标志物的表达。miR-451-5p结合ATF2并抑制其表达。过表达ATF2可逆转miR-451-5p对成骨分化的抑制作用。在成骨过程中，miR-451-5p通过抑制ATF2的功能负向调节细胞增殖和成骨。在临床上，miR-451-5p具有作为DFH早期预测的生物标志物的潜力。补充信息：在线版本包含补充资料，可在10.1007/s10616-026-00994-w获得。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cytotechnology 生物-生物工程与应用微生物

CiteScore

4.10

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The scope of the Journal includes: 1. The derivation, genetic modification and characterization of cell lines, genetic and phenotypic regulation, control of cellular metabolism, cell physiology and biochemistry related to cell function, performance and expression of cell products. 2. Cell culture techniques, substrates, environmental requirements and optimization, cloning, hybridization and molecular biology, including genomic and proteomic tools. 3. Cell culture systems, processes, reactors, scale-up, and industrial production. Descriptions of the design or construction of equipment, media or quality control procedures, that are ancillary to cellular research. 4. The application of animal/human cells in research in the field of stem cell research including maintenance of stemness, differentiation, genetics, and senescence, cancer research, research in immunology, as well as applications in tissue engineering and gene therapy. 5. The use of cell cultures as a substrate for bioassays, biomedical applications and in particular as a replacement for animal models.