Ultrasensitive, self-calibrating cortisol immunosensor enabled by dual-modal CuMOF-PEI@AuNPs@HRP immunoreceptor.

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2026-12-01 Epub Date: 2026-06-15 DOI:10.1016/j.talanta.2026.130164

Xinyi Wang, Minghao Wang, Wenbin Zhang, Dewang Yan, Liubing Kong, Gaofeng Wang

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引用次数: 0

Abstract

Accurate cortisol monitoring is essential for stress assessment and health management, yet reliable quantification remains challenging because signal fluctuations and matrix effects can compromise the performance of conventional single-mode immunosensors. Herein, we developed a fluorescence-colorimetric (FL-CL) dual-modal immunosensor based on a CuMOF-PEI@AuNPs@HRP (CPA) immunoreceptor for cortisol determination. The integrated sensing architecture generates correlated fluorescence and colorimetric responses from the same recognition event, which are combined through a normalized dual-signal output to improve calibration reliability. Under optimized conditions, the proposed sensor exhibited a detection limit of 0.22 ng/mL and a linear range of 3.1-200 ng/mL, covering physiologically relevant cortisol concentrations in saliva. Compared with single-modal assays and commercial kits, the proposed platform demonstrates enhanced sensitivity, improved correlation, good stability, and batch reproducibility. By integrating signal amplification, dual-modal readout, and simplified immunoreceptor fabrication within a single sensing platform, this work provides a reliable strategy for cortisol monitoring and offers potential for extension to other biomarker detection systems.

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超灵敏，自校准皮质醇免疫传感器启用双峰CuMOF-PEI@AuNPs@HRP免疫受体。

准确的皮质醇监测对于压力评估和健康管理至关重要，但可靠的量化仍然具有挑战性，因为信号波动和基质效应会损害传统单模免疫传感器的性能。在此，我们开发了一种基于CuMOF-PEI@AuNPs@HRP （CPA）免疫受体的荧光-比色（FL-CL）双峰免疫传感器，用于皮质醇的测定。集成传感架构从同一识别事件产生相关的荧光和比色响应，并通过归一化双信号输出组合，以提高校准可靠性。在优化条件下，该传感器的检测限为0.22 ng/mL，线性范围为3.1-200 ng/mL，涵盖了唾液中生理相关的皮质醇浓度。与单模态分析和商业试剂盒相比，该平台具有更高的灵敏度、更好的相关性、良好的稳定性和批量重现性。通过在单一传感平台内集成信号放大、双峰读出和简化的免疫受体制造，这项工作为皮质醇监测提供了可靠的策略，并提供了扩展到其他生物标志物检测系统的潜力。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.