Midgut microbial volatiles as potential behavioral regulator agents against virgin female Grapholita molesta

IF 4.4 1区农林科学 Q1 ENTOMOLOGY

Journal of Pest Science Pub Date : 2026-06-06 DOI:10.1007/s10340-026-02052-y

Xueli Wang, Minghui Yu, Jinyong Zhang, Hongtao Tu

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引用次数: 0

Abstract

Microorganisms residing in the insect gut play important roles in host nutrition, digestion, detoxification, and resistance to pathogens. However, the functions of gut microbial Volatile Organic Compounds (mVOCs) from insects remain largely unexplored. The oriental fruit moth, Grapholita molesta (Lepidoptera: Tortricidae), is recognized as one of the most destructive orchard pests affecting various Rosaceae fruit trees globally. In this study, we researched G. molesta and successfully isolated and identified eight bacterial and fungal strains from different genera present in the midgut of larvae and adults. Our findings indicated that both male and female adults of G. molesta were attracted to the ML10 and LAP strains, as demonstrated by Y-tube olfactometer assays. We subsequently analyzed their volatile compounds using Headspace Gas Chromatography-Ion Mobility Spectrometry (HS-GC-IMS). Y-tube olfactometer assays revealed that 2-methylpropanoic acid and 2-pentanone effectively attracted female adults at a concentration of 10 ppm. Additionally, heptaldehyde attracted female adults at concentrations of 0.1 ppm and 1 ppm. These findings expand the spectrum of volatile compounds that attract G. molesta and provide a foundation for developing microbe-based attractants for controlling this species.

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中肠微生物挥发物作为潜在的行为调节剂对未交配雌性鼹鼠的影响

寄生在昆虫肠道中的微生物在宿主的营养、消化、解毒和抵抗病原体等方面发挥着重要作用。然而，来自昆虫的肠道微生物挥发性有机化合物（mVOCs）的功能在很大程度上仍未被探索。东方果蛾（鳞翅目：蛾科）是公认的危害蔷薇科果树最具破坏性的果园害虫之一。在本研究中，我们成功地分离和鉴定了存在于幼虫和成虫中肠中不同属的8株细菌和真菌。结果表明，雌雄成虫均被ML10和LAP菌株所吸引，y管嗅探结果表明雌雄成虫均被ML10和LAP菌株所吸引。随后，我们使用顶空气相色谱-离子迁移谱法（HS-GC-IMS）分析了它们的挥发性化合物。y管嗅觉测定结果表明，2-甲基丙酸和2-戊酮在浓度为10ppm时能有效吸引雌成虫。此外，七醛以0.1 ppm和1ppm的浓度吸引雌性成虫。这些发现扩大了吸引鼠足虫的挥发性化合物的范围，为开发微生物引诱剂防治鼠足虫提供了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Pest Science 生物-昆虫学

CiteScore

10.40

自引率

8.30%

发文量

114

审稿时长

6-12 weeks

期刊介绍： Journal of Pest Science publishes high-quality papers on all aspects of pest science in agriculture, horticulture (including viticulture), forestry, urban pests, and stored products research, including health and safety issues. Journal of Pest Science reports on advances in control of pests and animal vectors of diseases, the biology, ethology and ecology of pests and their antagonists, and the use of other beneficial organisms in pest control. The journal covers all noxious or damaging groups of animals, including arthropods, nematodes, molluscs, and vertebrates. Journal of Pest Science devotes special attention to emerging and innovative pest control strategies, including the side effects of such approaches on non-target organisms, for example natural enemies and pollinators, and the implementation of these strategies in integrated pest management. Journal of Pest Science also publishes papers on the management of agro- and forest ecosystems where this is relevant to pest control. Papers on important methodological developments relevant for pest control will be considered as well.