Two-Trace Two-Dimensional (2T2D-COS) in the Analysis of Brain Tissue Sample Preparation Method.

IF 2.2 3区化学 Q2 INSTRUMENTS & INSTRUMENTATION

Applied Spectroscopy Pub Date : 2026-06-19 DOI:10.1177/00037028261465440

Emilia Wrona, Anna Błasiak, Gabriela Stopka, Aleksandra Nogaj, Aleksandra Kurek, Aleksandra Wesełucha-Birczyńska

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引用次数: 0

Abstract

Raman spectroscopy is widely used in biomedicine due to its non-invasive nature and the large amount of information it contains. This technique allows for the analysis of changes in lipid composition and concentration, which may be useful diagnostic parameter. These changes may appear at an early stage of neurodegenerative diseases and brain tumours. In addition, Raman spectroscopy allows measurements to be taken on tissues that have not been previously preserved. Therefore, it is important to investigate and select an appropriate method of sample storage in order to obtain Raman spectra of the highest possible quality. There are no clear guidelines in the currently available literature on how to prepare brain tissue samples to obtain the highest possible spectral quality. The aim of this study was to investigate the influence of an important parameter - the storage temperature of rat brain tissue on the quality of the spectrum. Based on a review of the literature, three methods of storing brain tissue were selected. The obtained Raman spectra were pre-processed and averaged. 2T-2D analysis was performed, comparing spectra differing in the way the tissue was stored before measurement. Based on the 2T-2D maps, differences were identified and discussed.

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EXPRESS：双微量二维（222d - cos）在脑组织样品分析中的制备方法。

拉曼光谱以其非侵入性和信息量大的特点在生物医学领域得到了广泛的应用。该技术允许分析脂质组成和浓度的变化，这可能是有用的诊断参数。这些变化可能出现在神经退行性疾病和脑肿瘤的早期阶段。此外，拉曼光谱允许对以前没有保存的组织进行测量。因此，为了获得尽可能高质量的拉曼光谱，研究和选择合适的样品存储方法是很重要的。关于如何制备脑组织样品以获得尽可能高的光谱质量，目前可用的文献中没有明确的指导方针。本研究的目的是探讨一个重要参数-大鼠脑组织的储存温度对光谱质量的影响。在查阅文献的基础上，我们选择了三种储存脑组织的方法。对得到的拉曼光谱进行预处理和平均。进行2T-2D分析，比较测量前组织存储方式的不同光谱。基于2T-2D地图，识别并讨论了差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Applied Spectroscopy 工程技术-光谱学

CiteScore

6.60

自引率

5.70%

发文量

139

审稿时长

3.5 months

期刊介绍： Applied Spectroscopy is one of the world''s leading spectroscopy journals, publishing high-quality peer-reviewed articles, both fundamental and applied, covering all aspects of spectroscopy. Established in 1951, the journal is owned by the Society for Applied Spectroscopy and is published monthly. The journal is dedicated to fulfilling the mission of the Society to “…advance and disseminate knowledge and information concerning the art and science of spectroscopy and other allied sciences.”