Development of Experimental in vitro Models to Elucidate Molecular Mechanisms Underlying Pathology and its Prevention.

Abstract

<b>Background and Objective:</b> Pathological processes at the cellular level can cause damage and understanding their underlining molecular mechanisms is crucial. The study aims to develop experimental <i>in vitro</i> models to investigate these mechanisms and identify potential strategies for preventing pathology. <b>Materials and Methods:</b> The average titers of gangliosides and anti-ganglioside antibodies in samples of non-malignant mouse embryonic cells 3T3 fibroblasts, of mouse malignant myeloma cells and mixed of the two cell types <i>in vitro</i>-cultures were assessed as homogenates of each one of the three types. The tested <i>in vitro</i>-cultures were applied in the role of experimental <i>in vitro</i>-models of patients with multifactor diseases in different phases, as well as of a healthy human organism. <b>Results:</b> The assessed varieties in the average titers of the gangliosides and anti-ganglioside antibodies could be due to participation of these molecules in different in various intra- and extra-cellular inter-molecular interactions. Production of antibodies/immunoglobulins by non-lymphoid and non-hematopoietic types of cells in appropriate conditions, but also about differentiation of non-immune and non-hematopoietic cell progenitors to myeloid and lymphoid directions in appropriate conditions, were proposed. <b>Conclusion:</b> Activation of internal protective mechanisms was suggested. Further studies should be performed.