Natural melanin nanoparticles modified with glycol chitosan for enhanced delivery of oxaliplatin.

IF 2.4 4区医学 Q3 ENGINEERING, BIOMEDICAL

Journal of Biomaterials Applications Pub Date : 2026-06-18 DOI:10.1177/08853282261460756

Güleycan Dedecengiz Varol, Miray Karamese, Seyma Uzum, Kaan Bayer, Rabia Selcen Kuru, Merve Güdül Bacanlı, Sevde Altuntas, Sakip Önder, Gizem Kaleli-Can

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Abstract

The aim of this study was to investigate the potential of natural melanin nanoparticles (MNPs) as carriers for oxaliplatin (OXP) delivery, with glycol chitosan (GC) surface modification to improve dispersion behavior and drug interaction. The isolated MNPs were spherical with an average diameter of 148 ± 28 nm, which increased to 261 ± 39 nm after GC coating. The particle size decreased to 148 ± 59 nm after OXP loading. Physicochemical characterization confirmed surface modification and drug association. The OXP-loaded GC-MNP formulation showed a loading efficiency of 67.8 ± 4.6% and a biphasic release profile, with an initial rapid release followed by complete drug release within 24 h. Release kinetic analysis showed the best fit with the first-order model, indicating concentration-dependent release behavior. Biological studies demonstrated time-dependent responses in MCF-7 cells. All tested formulations showed low cytotoxicity at 6 h. However, OXP-loaded GC-MNP exhibited higher cytotoxicity than free OXP under selected conditions. Furthermore, concentration-dependent genotoxic effects were observed in comet assay experiments. These findings suggest that GC-coated melanin nanoparticles may serve as a promising carrier platform for short-term modulation of OXP delivery in breast cancer applications.

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用乙二醇壳聚糖修饰的天然黑色素纳米颗粒增强奥沙利铂的递送。

本研究的目的是研究天然黑色素纳米颗粒（MNPs）作为奥沙利铂（OXP）递送载体的潜力，并通过乙二醇壳聚糖（GC）表面改性来改善分散行为和药物相互作用。分离得到的MNPs为球形，平均直径为148±28 nm，经GC包覆后增大到261±39 nm。OXP加载后，颗粒尺寸减小至148±59 nm。物理化学表征证实了表面修饰和药物关联。oxp - mnp的加载效率为67.8±4.6%，呈两相释放，初始快速释放，24 h内完全释放。释放动力学分析表明，该处方符合一级模型，具有浓度依赖性释放行为。生物学研究证实MCF-7细胞有时间依赖性反应。所有测试的配方在6 h时均表现出较低的细胞毒性。然而，在特定条件下，负载OXP的GC-MNP表现出比游离OXP更高的细胞毒性。此外，浓度依赖性的基因毒性效应在彗星试验中被观察到。这些发现表明，气相色谱包被的黑色素纳米颗粒可能作为一种有前途的载体平台，用于短期调节OXP在乳腺癌中的传递。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Biomaterials Applications 工程技术-材料科学：生物材料

CiteScore

5.10

自引率

3.40%

发文量

144

审稿时长

1.5 months

期刊介绍： The Journal of Biomaterials Applications is a fully peer reviewed international journal that publishes original research and review articles that emphasize the development, manufacture and clinical applications of biomaterials. Peer-reviewed articles by biomedical specialists from around the world cover: New developments in biomaterials, R&D, properties and performance, evaluation and applications Applications in biomedical materials and devices - from sutures and wound dressings to biosensors and cardiovascular devices Current findings in biological compatibility/incompatibility of biomaterials The Journal of Biomaterials Applications publishes original articles that emphasize the development, manufacture and clinical applications of biomaterials. Biomaterials continue to be one of the most rapidly growing areas of research in plastics today and certainly one of the biggest technical challenges, since biomaterial performance is dependent on polymer compatibility with the aggressive biological environment. The Journal cuts across disciplines and focuses on medical research and topics that present the broadest view of practical applications of biomaterials in actual clinical use. The Journal of Biomaterial Applications is devoted to new and emerging biomaterials technologies, particularly focusing on the many applications which are under development at industrial biomedical and polymer research facilities, as well as the ongoing activities in academic, medical and applied clinical uses of devices.