Rapid identification directly from urine using QUICKprep kit.

Abstract

Urinary tract infections are common, mainly caused by E. coli. Conventional diagnosis based on culture, delays result for 48 h, promoting empirical, often inadequate, treatments. This study validated the QUICKprep^® (FASTinov^®) kit, which concentrates viable microbial cells and removes debris in under five minutes for MALDI-TOF-MS identification. A total of 281 positive urine samples, screened positive on Sysmex, were tested. In inoculated samples, results were fully concordant with culture; in clinical samples, 13/155 were not identified by MALDI-TOF-MS. QUICKprep^® enabled accurate identification direct from urine in around 15 min, offering faster diagnosis, potential for antimicrobial testing, and improved therapeutic decisions.