The influence of haemorrhage and fluid resuscitation with either Plasma-Lyte A or Ringer’s Lactate in Beagle dogs under sevoflurane anaesthesia—part 2: Buccal mucosal microcirculation

IF 2.5 2区农林科学 Q2 VETERINARY SCIENCES

Veterinary anaesthesia and analgesia Pub Date : 2026-07-01 Epub Date: 2026-04-16 DOI:10.1016/j.vaa.2026.101234

Christina A Gruell, Annette PN Kutter, Federico Gianolli, Barbara Steblaj

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引用次数: 0

Abstract

Objective

To compare the effects of haemorrhage and subsequent fluid resuscitation with Plasma-Lyte A (PA) or Ringer’s Lactate (RL) on buccal microcirculation.

Study design

Single-centre, randomised, blinded, experimental study.

Animals

A group of 12 adult, healthy, intact, purpose-bred Beagle dogs.

Methods

In sevoflurane anaesthetised Beagle dogs, a sidestream dark field (SDF) imaging device was positioned on the buccal mucosa to evaluate microcirculatory variables, which included perfused De Backer density (pDBD) and proportion of perfused vessels. Videos were recorded at baseline, following the removal of 30 mL kg^-1 of blood to induce hypovolaemia (HV), and after each 10 mL kg^-1 intravenous fluid bolus (B1 and B2) according to the assigned treatment group. Data were compared between groups and over time using a linear mixed model with group and time point as fixed and dogs as random factors. Post hoc multiple comparisons were performed with Sidak’s corrections. A p value < 0.05 was considered significant.

Results

The haemorrhage resuscitation model resulted in significant changes in microcirculation over time. In group RL, pDBD was significantly more reduced compared with group PA at HV [RL: 1.3 (0.9–1.8) n mm^-1 (median ± 95% confidence interval), PA: 2.3 (1.6–2.7) n mm^-1, p = 0.004] and B2 [RL: 2 (1.7–2.7), PA: 3.3 (2.7– 4.2), p < 0.001]. Proportion of perfused vessels was also more reduced in group RL compared with group PA at HV [RL: 57 (53–77)%, PA: 78 (69–93)%, p < 0.001] and B2 [RL: 72 (65–96)%, PA: 85 (77–99)%, p = 0.012].

Conclusions and clinical relevance

Dogs administered PA showed fewer changes in pDBD and proportion of perfused vessels values after haemorrhage and resuscitation with 20 mL kg^-1 PA compared with those administered RL. Due to the small sample size these preliminary findings warrant further research.

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七氟醚麻醉对Beagle犬出血及液体复苏的影响(2)：口腔黏膜微循环。

目的：比较出血及术后液体复苏对口腔微循环的影响。研究设计：单中心、随机、盲法实验研究。动物：一组12只健康、完整的成年比格犬。方法：在七氟醚麻醉的Beagle犬的颊粘膜上放置侧流暗场（SDF）成像装置，评估微循环变量，包括灌注De Backer密度（pDBD）和灌注血管比例。在基线时，在抽取30ml kg-1的血液诱导低血容量（HV）后，以及根据指定的治疗组每次10 mL kg-1静脉输液（B1和B2）后，记录视频。使用线性混合模型对组间数据和时间进行比较，其中组和时间点为固定因素，狗为随机因素。采用Sidak校正法进行事后多重比较。p值< 0.05被认为是显著的。结果：随着时间的推移，出血复苏模型导致微循环发生显著变化。与PA组相比，RL组在HV [RL: 1.3 (0.9 ~ 1.8) n mm-1（中位数±95%可信区间），PA: 2.3 (1.6 ~ 2.7) n mm-1, p = 0.004]和B2 [RL: 2 (1.7 ~ 2.7), PA: 3.3 (2.7 ~ 4.2), p < 0.001]时pDBD明显降低。与PA组相比，RL组在HV组灌注血管比例减少较多[RL: 57 (53-77)%, PA: 78 (69-93)%, p < 0.001]， B2组[RL: 72 (65-96)%, PA: 85 (77-99)%, p = 0.012]。结论及临床意义：与给予RL的狗相比，给予PA的狗在20 mL kg-1 PA出血和复苏后pDBD和灌注血管值比例的变化更小。由于样本量小，这些初步发现值得进一步研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary anaesthesia and analgesia 农林科学-兽医学

CiteScore

3.10

自引率

17.60%

发文量

审稿时长

97 days

期刊介绍： Veterinary Anaesthesia and Analgesia is the official journal of the Association of Veterinary Anaesthetists, the American College of Veterinary Anesthesia and Analgesia and the European College of Veterinary Anaesthesia and Analgesia. Its purpose is the publication of original, peer reviewed articles covering all branches of anaesthesia and the relief of pain in animals. Articles concerned with the following subjects related to anaesthesia and analgesia are also welcome: the basic sciences; pathophysiology of disease as it relates to anaesthetic management equipment intensive care chemical restraint of animals including laboratory animals, wildlife and exotic animals welfare issues associated with pain and distress education in veterinary anaesthesia and analgesia. Review articles, special articles, and historical notes will also be published, along with editorials, case reports in the form of letters to the editor, and book reviews. There is also an active correspondence section.