Low Intensity Pulsed Ultrasound Alleviates the Pain of Monosodium Iodoacetate-Induced Osteoarthritis in Rats

IF 2.1 4区医学 Q2 ACOUSTICS

Journal of Ultrasound in Medicine Pub Date : 2026-10-13 Epub Date: 2026-06-17 DOI:10.1002/jum.70339

Zhihua Liu MM, Yang Dong MM, Peng Xu MM, Xia Chen MM

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引用次数: 0

Abstract

Objectives

Osteoarthritis pain is a common chronic pain. Low intensity pulsed ultrasound (LIPUS) is a non-invasive treatment commonly used for musculoskeletal system diseases, but it is unclear whether it can alleviate osteoarthritis (OA) pain. The aim of this study is to investigate the therapeutic effects of LIPUS on pain relief and joint repair in OA rat models, and to explore its potential mechanisms.

Methods

Twenty-four Sprague–Dawley rats were divided into Sham, monosodium iodoacetate (MIA), MIA + LIPUS, and MIA + Sham LIPUS groups. OA was induced via intra-articular MIA injection. LIPUS was administered for 20 minutes/14 days. Behavioral tests, quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR), histopathological, and immunohistochemical assessments were conducted.

Results

LIPUS significantly increased paw withdrawal thresholds (PWT) and thermal withdrawal latencies (PWL), improved gait symmetry, and enhanced total walking distance. Piezo1, Piezo2, Trpv1, and Calca (encoding CGRP) mRNA levels in dorsal root ganglia were downregulated. Histopathological and immunohistochemical analysis revealed that LIPUS treatment preserved cartilage matrix integrity, reduced cartilage surface erosion, decreased Mankin scores, and promoted cartilage regeneration.

Conclusion

LIPUS significantly alleviated OA-induced pain and joint damage in rats by downregulating mRNA expression of pain-related molecules and promoting cartilage preservation. These findings suggested that LIPUS has the potential to be a non-invasive, non-pharmacological therapy for OA, offering a promising alternative to conventional treatments.

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低强度脉冲超声减轻碘酸钠诱导的骨关节炎大鼠疼痛。

目的：骨关节炎疼痛是一种常见的慢性疼痛。低强度脉冲超声（LIPUS）是一种通常用于肌肉骨骼系统疾病的非侵入性治疗方法，但它是否能缓解骨关节炎（OA）疼痛尚不清楚。本研究旨在探讨LIPUS对OA大鼠模型疼痛缓解和关节修复的治疗作用，并探讨其可能的机制。方法：将24只sd大鼠分为假手术组、碘乙酸单钠组、MIA + LIPUS组和MIA + Sham LIPUS组。通过关节内注射MIA诱导OA。LIPUS给药20分钟/14天。进行行为测试、定量实时逆转录聚合酶链反应（qRT-PCR）、组织病理学和免疫组织化学评估。结果：LIPUS显著提高足爪戒断阈值（PWT）和热戒断潜伏期（PWL），改善步态对称性，增加总步行距离。背根神经节中Piezo1、Piezo2、Trpv1和Calca（编码CGRP） mRNA水平下调。组织病理学和免疫组织化学分析显示，LIPUS治疗能保持软骨基质的完整性，减少软骨表面侵蚀，降低Mankin评分，促进软骨再生。结论：LIPUS通过下调疼痛相关分子mRNA表达，促进软骨保存，显著减轻oa诱导的大鼠疼痛和关节损伤。这些发现表明LIPUS有潜力成为OA的一种非侵入性、非药物治疗方法，为传统治疗提供了一种有希望的替代方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Ultrasound in Medicine 医学-核医学

CiteScore

5.10

自引率

4.30%

发文量

205

审稿时长

1.5 months

期刊介绍： The Journal of Ultrasound in Medicine (JUM) is dedicated to the rapid, accurate publication of original articles dealing with all aspects of medical ultrasound, particularly its direct application to patient care but also relevant basic science, advances in instrumentation, and biological effects. The journal is an official publication of the American Institute of Ultrasound in Medicine and publishes articles in a variety of categories, including Original Research papers, Review Articles, Pictorial Essays, Technical Innovations, Case Series, Letters to the Editor, and more, from an international bevy of countries in a continual effort to showcase and promote advances in the ultrasound community. Represented through these efforts are a wide variety of disciplines of ultrasound, including, but not limited to: -Basic Science- Breast Ultrasound- Contrast-Enhanced Ultrasound- Dermatology- Echocardiography- Elastography- Emergency Medicine- Fetal Echocardiography- Gastrointestinal Ultrasound- General and Abdominal Ultrasound- Genitourinary Ultrasound- Gynecologic Ultrasound- Head and Neck Ultrasound- High Frequency Clinical and Preclinical Imaging- Interventional-Intraoperative Ultrasound- Musculoskeletal Ultrasound- Neurosonology- Obstetric Ultrasound- Ophthalmologic Ultrasound- Pediatric Ultrasound- Point-of-Care Ultrasound- Public Policy- Superficial Structures- Therapeutic Ultrasound- Ultrasound Education- Ultrasound in Global Health- Urologic Ultrasound- Vascular Ultrasound