One-step lipid–polymeric hybrid nanoparticles as intracanal drug delivery system: Synthesis, characterization, antimicrobial activity, and dentinal penetration

IF 4.3 2区医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL

Nanomedicine : nanotechnology, biology, and medicine Pub Date : 2026-07-01 Epub Date: 2026-06-16 DOI:10.1016/j.nano.2026.102979

Ahmad AlMukhallalati, Amal Yousfan, Diego Hidalgo-Martínez, Samer Ibrahim, Khitam Al Moarrawi, Abdulsamie Hanano

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Abstract

Introduction

This study developed two lipid–polymeric hybrid nanoparticle thermosensitive gels, hypothesizing enhanced physicochemical properties, antimicrobial efficacy, and dentinal penetration compared to free drugs.

Methods

Ciprofloxacin-loaded lecithin–PLGA nanoparticles (CIP-LPNPs) and chitosan–lecithin nanoparticles (CS-LNPs) were synthesized via nanoprecipitation and characterized by DLS, SEM, FTIR and HPLC. Antimicrobial activity against Enterococcus faecalis, Escherichia coli and Pseudomonas aeruginosa was assessed by disk diffusion, MIC and biofilm assays; dentinal penetration of FITC-labeled CS-LNPs was evaluated by confocal microscopy.

Results

CIP-LPNPs and CS-LNPs measured 140.6 and 130.9 nm with zeta potentials of −29.4 and +28.9 mV, respectively. CIP-LPNPs showed 55.7% encapsulation and biphasic release. Both nanoparticles enhanced biofilm eradication against all strains; CS-LNPs exhibited two-fold lower MICs and significantly deeper tubular penetration than free chitosan (p < 0.0001).

Conclusions

Both CIP-LPNPs and CS-LNPs improved physicochemical and antibiofilm properties over their free drugs; however, as no biocompatibility assessment was performed, these formulations cannot be considered safe without such validation.

Abstract Image

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一步脂质-聚合物杂化纳米颗粒作为肛管内给药系统：合成、表征、抗菌活性和牙本质渗透。

本研究开发了两种脂质-聚合物杂化纳米颗粒热敏凝胶，假设与游离药物相比，其物理化学性能、抗菌功效和牙本质穿透性都有所提高。方法：采用纳米沉淀法合成环丙沙星负载的卵磷脂- plga纳米颗粒（cip - lnps）和壳聚糖-卵磷脂纳米颗粒（CS-LNPs），并采用DLS、SEM、FTIR和HPLC对其进行表征。采用纸片扩散法、MIC法和生物膜法检测对粪肠球菌、大肠杆菌和铜绿假单胞菌的抑菌活性；用共聚焦显微镜观察fitc标记的CS-LNPs的牙本质渗透情况。结果：cip - lnps和CS-LNPs的波长分别为140.6和130.9 nm， zeta电位分别为-29.4和+28.9 mV。cip - lnps包封率为55.7%，双相释放。这两种纳米颗粒都增强了对所有菌株的生物膜根除；与游离壳聚糖相比，CS-LNPs的mic低两倍，管状穿透深度也明显加深（p ）。结论：cip - lnp和CS-LNPs的理化性能和抗生物膜性能都优于游离壳聚糖；然而，由于没有进行生物相容性评估，没有这样的验证，这些配方不能被认为是安全的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nanomedicine : nanotechnology, biology, and medicine 工程技术-纳米科技

CiteScore

11.10

自引率

0.00%

发文量

133

审稿时长

42 days

期刊介绍： The mission of Nanomedicine: Nanotechnology, Biology, and Medicine (Nanomedicine: NBM) is to promote the emerging interdisciplinary field of nanomedicine. Nanomedicine: NBM is an international, peer-reviewed journal presenting novel, significant, and interdisciplinary theoretical and experimental results related to nanoscience and nanotechnology in the life and health sciences. Content includes basic, translational, and clinical research addressing diagnosis, treatment, monitoring, prediction, and prevention of diseases.