Moleculartyping and antibiotic resistance pattern of Aeromonas hydrophila isolated from children with diarrhea in Qom, Iran.

IF 1.9 Q4 MICROBIOLOGY

Iranian Journal of Microbiology Pub Date : 2026-06-01 DOI:10.18502/ijm.v18i3.21655

Pegah Shakib, Mohammad Reza Zolfaghari, Mina Hosseini, Fatemeh Mosaei, Seyed Soheil Aghaei

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引用次数: 0

Abstract

Background and objectives: Today there are reports of the spread of multiple antibiotic resistance in Aeromonas spp., which can be opportunistic pathogens and can cause infection in the host. This study aimed to investigate ERIC-PCR and BOX-PCR typing, drug resistance pattern, and tetracycline, sulfonamide, and fluoroquinolone resistance genes in Aeromonas hydrophila collected from diarrhea samples of children in Qom City, Iran.

Materials and methods: In our cross-sectional descriptive study, isolates of A. hydrophila isolated from children's diarrhea samples at Hazrat Masoumeh Hospital in Qom were evaluated between 2018 and 2020. Once isolates confirmed, the level of antimicrobial resistance of the strains was determined by the disk diffusion assay following the recommendations of Clinical and Laboratory Standards Institute (CLSI). The genes of tetA, tetB, tetC, tetD, tetE, sul1, sul2, Edq1qc, qnrA, qnrB, qnrS were studied using the PCR method. Then the genetic diversity of the isolates was investigated by ERIC-PCR and box-PCR. Finally, data analysis was done with SPSS version 16.

Results: From 100 A. hydrophila strains, the highest resistance, and sensitivity were related to ampicillin (92%) and azithromycin (96%), respectively. The frequency of tetA, tetB, tetC, tetD, tetE, sul1, sul2, Edq1qc, qnrA, qnrB, qnrS genes was 23, 24, 27, 23, 21, 26, 10, 22, 8, 11, 15%, respectively. With ERIC-PCR and BOX-PCR techniques, all isolates could be typed, so that 8 genetic patterns were determined in ERIC-PCR and 10 genetic patterns were determined in BOX-PCR. A significant relationship was found between cefotaxime and tetA (p = 0.012), ciprofloxacin and tetC (p = 0.018), cefotaxime and qnrA (p = 0.04), and also between tetracycline resistance genes and both sulfonamide genes (p > 0.05).

Conclusion: For the first time this study revealed resistance genes for tetracycline, sulfonamide, and fluoroquinolones in A. hydrophila isolated from childeren's diarrhea samples in Qom. Even though these antimicrobial resistance genes are present in our study and bacteria are resistant to different types of antimicrobials, it can be predicted that the abundance of these genes will increase in the future. We also observed that A. hydrophila isolates had high genetic diversity.

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伊朗库姆地区腹泻患儿嗜水气单胞菌分子分型及耐药模式

背景和目的：目前有报道称气单胞菌具有多种抗生素耐药性，它可能是机会致病菌，并可引起宿主感染。本研究旨在了解伊朗库姆市儿童腹泻标本中嗜水气单胞菌的ERIC-PCR和BOX-PCR分型、耐药模式以及四环素、磺胺和氟喹诺酮类药物耐药基因。材料和方法：在我们的横断面描述性研究中，对2018年至2020年库姆Hazrat Masoumeh医院儿童腹泻样本中分离的嗜水单杆菌进行了评估。一旦确认分离株，根据临床和实验室标准协会（CLSI）的建议，采用圆盘扩散法确定菌株的抗微生物药物耐药性水平。采用PCR方法对tetA、tetB、tetC、tetD、tetE、sul1、sul2、Edq1qc、qnrA、qnrB、qnrS基因进行了分析。采用ERIC-PCR和box-PCR分析分离菌株的遗传多样性。最后，使用SPSS version 16进行数据分析。结果：100株嗜水单胞杆菌对氨苄西林（92%）和阿奇霉素（96%）的耐药性和敏感性最高。tetA、tetB、tetC、tetD、tetE、sul1、sul2、Edq1qc、qnrA、qnrB、qnrS基因的频率分别为23、24、27、23、21、26、10、22、8、11、15%。采用ERIC-PCR和BOX-PCR技术对所有分离株进行分型，ERIC-PCR鉴定出8种遗传型，BOX-PCR鉴定出10种遗传型。头孢噻肟与tetA （p = 0.012）、环丙沙星与tetC （p = 0.018）、头孢噻肟与qnrA （p = 0.04）、四环素耐药基因与两种磺胺类基因之间存在显著相关（p < 0.05）。结论：本研究首次揭示了库姆地区儿童腹泻标本中分离的嗜水单胞菌对四环素、磺胺和氟喹诺酮类药物的耐药基因。尽管在我们的研究中存在这些抗微生物药物抗性基因，并且细菌对不同类型的抗微生物药物具有耐药性，但可以预测这些基因的丰度将在未来增加。我们还观察到嗜水单胞菌分离株具有较高的遗传多样性。

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来源期刊

Iranian Journal of Microbiology MICROBIOLOGY-

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.