Molecular detection of Anaplasma spp. in blood and ticks collected from sheep and goat in West Azerbaijan province, Iran.

IF 1.9 Q4 MICROBIOLOGY

Iranian Journal of Microbiology Pub Date : 2026-06-01 DOI:10.18502/ijm.v18i3.21666

Ahmad Enferadi, Amir Tukmechi, Katayoon Nofouzi, Zahraalsadat Tavakolian

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引用次数: 0

Abstract

Background and objectives: Anaplasmosis, an ailment affecting both captive and free-ranging small ruminants, is instigated by Anaplasma spp., a tick-vectored, obligate intracellular rickettsial bacterium. Iran's ovine and caprine populations, numbering roughly 71 million, are vital to its financial structure.

Materials and methods: This study investigated the prevalence of Anaplasma species in sheep and goats within West Azerbaijan province. Additionally, nucleic acid specimens were isolated from gathered ticks and examined for Anaplasma spp. Through polymerase chain reaction (PCR) utilizing the major surface protein gene (groEL).

Results: Anaplasma was detected in 161 (69.0%) of 919 ovine and 82 (71.3%) of 243 caprine blood DNA extracts. Subsequently, genetic material from 426 ticks comprised of Rhipicephalus sanguineus (n=146), Rhipicephalus turanicus (n=63), Hyalomma asiaticum (n=56), Hyalomma anatolicum (n=74), and Hyalomma egebtiom (n=87) was screened for A. ovis utilizing the same methodology.

Conclusion: This research not only confirmed the presence of A. ovis within Iranian sheep and goats but also implicated ticks as a possible vector for its transmission. The findings emphasize the importance of monitoring the health status of Iran's small ruminants to detect clinical manifestations of anaplasmosis and of implementing effective tick control strategies worldwide.

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伊朗西阿塞拜疆省绵羊和山羊采集的血液和蜱中无原体的分子检测。

背景和目的：无形体病是一种影响圈养和自由放养的小反刍动物的疾病，由一种蜱媒、专性细胞内立克次体细菌无形体引起。伊朗的绵羊和山羊数量约为7100万只，对其金融结构至关重要。材料和方法：本研究调查了西阿塞拜疆省绵羊和山羊无原体的流行情况。此外，从采集的蜱中分离核酸标本，利用主要表面蛋白基因（groEL）进行聚合酶链反应（PCR）检测无原体。结果：919只羊的161份（69.0%）和243只羊的82份（71.3%）血液DNA提取液中检出无原体。随后，利用相同的方法，从426只蜱（包括血头蜱（146只）、图头蜱（63只）、亚洲透明眼蜱（56只）、鸭头透明眼蜱（74只）和卵头透明眼蜱（87只））中筛选了卵头蜱的遗传物质。结论：本研究不仅证实伊朗绵羊和山羊中存在鹅伊螨，而且提示蜱虫可能是其传播媒介。研究结果强调了监测伊朗小反刍动物健康状况的重要性，以发现无形体病的临床表现，并在世界范围内实施有效的蜱虫控制战略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Microbiology MICROBIOLOGY-

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.